Abdulrazak Nuhu, Asiya Umar Imam, Usman NataaLa Shehu, Unata Iduh Micheal, Farida Aminu
Department of Medical Microbiology, Usmanu Danfodiyo University Sokoto, Nigeria.
Department of chemical pathology, Usmanu Danfodiyo University Sokoto, Nigeria.
J Intercult Ethnopharmacol. 2015 Apr-Jun;4(2):96-101. doi: 10.5455/jice.20141231014333. Epub 2015 Jan 20.
This study assessed within 4 days of suppressive test in vivo antimalarial activity of Ethanolic extract of root and stem bark of Cassia sieberiana DC against chloroquine sensitive strain of Plasmodium berghei NK65 in mice.
Two sets, each of five groups of four mice per each group were used. The groups of animals were administered with 100, 200, and 300 mg extract/kg body weight respectively, while positive control group were administered with 5 mg chloroquine/kg body weight and the negative control, were administered with 5 m1 distilled water/kg body weight. Oral acute toxicity was evaluated using up and down procedure.
Both the root and stem bark extract of C. sieberiana showed antimalarial property for suppressive tests. Chemo suppression of the root extract exerted significant (P < 0.05) dose-dependent reduction in the level of parasiteamia of 30.7%, 52.7%, and 55.8%. And from stem extract 17.6%, 38.0%, and 63.9% were recorded on mice when compared with 96.0% suppressive rate obtained from weight of chloroquine. The phytochemical screening of the plants root and stem bark extract revealed the presence of alkaloids, anthraquinones, flavonoids, triterpenoids, tannins, cardiac glycosides, saponins, reducing sugars and carbohydrates. The oral median lethal dose was determined to be >3000 mg/kg body weight.
The acute toxicity results of this study showed that the plant parts used are assumed to be safe and has anti-plasmodial activity that can be explored for the management of malaria.
本研究在体内抑制试验的4天内，评估了腊肠树（Cassia sieberiana DC）根和茎皮乙醇提取物对小鼠体内伯氏疟原虫NK65氯喹敏感株的抗疟活性。
使用两组，每组五只小鼠，分为五个小组。分别给动物组施用100、200和300毫克提取物/千克体重，而阳性对照组施用5毫克氯喹/千克体重，阴性对照组施用5毫升蒸馏水/千克体重。使用上下法评估口服急性毒性。
腊肠树的根和茎皮提取物在抑制试验中均显示出抗疟特性。根提取物的化学抑制作用使疟原虫血症水平显著（P<0.05）呈剂量依赖性降低，分别为30.7%、52.7%和55.8%。与氯喹重量获得的96.0%抑制率相比，茎提取物在小鼠身上的抑制率分别为17.6%、38.0%和63.9%。对植物根和茎皮提取物进行的植物化学筛选显示存在生物碱、蒽醌、黄酮类、三萜类、单宁、强心苷、皂苷、还原糖和碳水化合物。经测定，口服半数致死剂量>3000毫克/千克体重。
本研究的急性毒性结果表明，所使用的植物部位被认为是安全的，并且具有抗疟原虫活性，可用于疟疾的治疗。
