Department of Orthopaedics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, 453100, Henan, China.
Department of Orthopaedics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
Biomed Pharmacother. 2017 Aug;92:437-444. doi: 10.1016/j.biopha.2017.05.081. Epub 2017 May 27.
Long non-coding RNAs (lncRNAs) have been strongly associated with various types of cancer. this study was to explore the critical role of lncRNA HOXA11-AS in osteosarcoma (OS) progression. Briefly, we should that the expression of HOXA11-AS was upregulated in OS tissues and cell lines. The high expression of HOXA11-AS was associated with advanced clinical stage, distant metastasis and poor overall survival of OS. In addition, We found that HOXA11-AS silencing suppressed OS cells proliferation, invasion and induced cell arrest in G0/G1 phase. Furthermore, our data showed that HOXA11-AS acts as an endogenous sponge by directly binding miR-124-3p, and decreasing the expression of miR-124-3p. Moreover, we found that HOXA11-AS may regulate tumor progression by affecting miR-124-3p targets, and ROCK1 expression. To conclude, our study helps to elucidate the effectiveness of HOXA11-AS promotion on OS cell proliferation and metastasis. A better understanding of interaction mechanism between HOXA11-AS-miR-124-3p-ROCK1 signaling axis may be a step forward in the development of new therapeutic strategies for the treatment of OS.
长链非编码 RNA（lncRNA）与多种类型的癌症密切相关。本研究旨在探讨 lncRNA HOXA11-AS 在骨肉瘤（OS）进展中的关键作用。简而言之，我们发现 HOXA11-AS 在 OS 组织和细胞系中表达上调。HOXA11-AS 的高表达与 OS 的晚期临床分期、远处转移和不良总生存期相关。此外，我们发现 HOXA11-AS 沉默抑制了 OS 细胞的增殖、侵袭，并诱导细胞停滞在 G0/G1 期。此外，我们的数据表明，HOXA11-AS 作为内源性海绵体，直接与 miR-124-3p 结合，降低 miR-124-3p 的表达。此外，我们发现 HOXA11-AS 可能通过影响 miR-124-3p 靶点和 ROCK1 表达来调节肿瘤进展。总之，我们的研究有助于阐明 HOXA11-AS 促进 OS 细胞增殖和转移的有效性。更好地理解 HOXA11-AS-miR-124-3p-ROCK1 信号轴的相互作用机制可能是开发治疗 OS 的新治疗策略的重要一步。
