Medveczky I, Kovács L, Kovács F, Papp L
Department of Epizootiology, University of Veterinary Sciences, Budapest, Hungary.
Med Vet Entomol. 1988 Jan;2(1):81-6. doi: 10.1111/j.1365-2915.1988.tb00052.x.
Starved houseflies were held over a suspension of Aujeszky's virus (PRV-1) for 24-48 h. One group was rinsed in 70% ethanol to kill virus attached to the body surface. No virus was isolated from this group. For the other group the titre of virus decreased more rapidly on the body surface of flies than in the environment. Model experiments demonstrated that the Aujeszky's virus cannot survive in the body of the housefly but the body surface may be contaminated for a period of time depending on the initial viral titre. Experiments showed that susceptible pigs fed on flies contaminated with Aujeszky's virus may become infected. The quantity of virus (5 x 10(5) pfu ml-1) shed by a single housefly during biting and vomiting on the cornea or abraded skin proved to be sufficient to cause infection in susceptible pigs, rabbits and a lamb. It is possible that houseflies could play a role in transmission of infection within herds. Transmission between herds is much less likely.
将饥饿的家蝇置于奥耶斯基病毒(PRV - 1)悬液上方24至48小时。一组家蝇用70%乙醇冲洗以杀死附着在体表的病毒。该组未分离到病毒。对于另一组,家蝇体表病毒滴度的下降速度比环境中更快。模型实验表明,奥耶斯基病毒不能在家蝇体内存活,但体表可能会在一段时间内被污染,这取决于初始病毒滴度。实验表明,食用被奥耶斯基病毒污染的家蝇的易感猪可能会被感染。事实证明,一只家蝇在叮咬和呕吐到角膜或擦伤皮肤时所释放的病毒量(5×10⁵ pfu ml⁻¹)足以在易感猪、兔子和一只羔羊中引起感染。家蝇有可能在畜群内感染传播中起作用。而在畜群间传播的可能性则小得多。