Mishra Vartika, Jana Asim K, Jana Mithu Maiti, Gupta Antriksh
Department of Biotechnology, Dr B R A National Institute of Technology, G T Road Bye Pass, Jalandhar, Punjab, 144011, India.
Department of Chemistry, Dr B R A National Institute of Technology, Jalandhar, Punjab, 144011, India.
3 Biotech. 2017 Jun;7(2):110. doi: 10.1007/s13205-017-0719-4. Epub 2017 May 31.
Sweet sorghum bagasse (SSB) from food processing and agricultural industry has attracted the attention for uses in production of biofuel, enzymes and other products. The alteration in lignocellulolytic enzymes by use of supplements in fungal pretreatment of SSB to achieve higher lignin degradation, selectivity value and enzymatic hydrolysis to fermentable sugar was studied. Fungal strain Coriolus versicolor was selected for pretreatment due to high ligninolytic and low cellulolytic enzyme production resulting in high lignin degradation and selectivity value. SSB was pretreated with supplements of veratryl alcohol, syringic acid, catechol, gallic acid, vanillin, guaiacol, CuSO and MnSO. The best results were obtained with CuSO, gallic acid and syringic acid supplements. CuSO increased the activities of laccase (4.9-fold) and polyphenol oxidase (1.9-fold); gallic acid increased laccase (3.5-fold) and manganese peroxidase (2.5-fold); and syringic acid increased laccase (5.6-fold), lignin peroxidase (13-fold) and arylalcohol oxidase (2.8-fold) resulting in enhanced lignin degradations and selectivity values than the control. Reduced cellulolytic enzyme activities resulted in high cellulose recovery. Enzymatic hydrolysis of pretreated SSB yielded higher sugar due to degradation of lignin and reduced the crystallinity of cellulose. The study showed that supplements could be used to improve the pretreatment process. The results were confirmed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and thermogravimetric/differential thermogravimetric analysis of SSB.
食品加工和农业产业产生的甜高粱渣（SSB）在生物燃料、酶及其他产品生产中的应用已受到关注。研究了在SSB的真菌预处理中使用添加剂对木质纤维素酶的影响，以实现更高的木质素降解、选择性值以及将其酶解为可发酵糖。由于云芝产生的木质素分解酶高而纤维素分解酶低，导致木质素降解率高和选择性值高，因此选择云芝菌株进行预处理。用藜芦醇、丁香酸、儿茶酚、没食子酸、香草醛、愈创木酚、硫酸铜和硫酸锰对SSB进行预处理。硫酸铜、没食子酸和丁香酸添加剂取得了最佳效果。硫酸铜使漆酶活性提高了4.9倍，多酚氧化酶活性提高了1.9倍；没食子酸使漆酶活性提高了3.5倍，锰过氧化物酶活性提高了2.5倍；丁香酸使漆酶活性提高了5.6倍，木质素过氧化物酶活性提高了13倍，芳醇氧化酶活性提高了2.8倍，从而使木质素降解和选择性值比对照有所提高。纤维素分解酶活性降低导致纤维素回收率提高。预处理后的SSB进行酶水解，由于木质素的降解和纤维素结晶度的降低，产生了更高的糖产量。研究表明，添加剂可用于改进预处理工艺。通过对SSB的扫描电子显微镜、X射线衍射、傅里叶变换红外光谱和热重/微商热重分析证实了结果。
