Da Silva Pinto Edlaine Acosta, Garcia Edariane Menestrino, de Almeida Krissia Aparecida, Fernandes Caroline Feijó Lopes, Tavella Ronan Adler, Soares Maria Cristina Flores, Baisch Paulo Roberto Martins, Muccillo-Baisch Ana Luíza, da Silva Júnior Flavio Manoel Rodrigues
Laboratório de Ensaios Farmacológicos e Toxicológicos, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália, km 8, Campus Carreiros, Rio Grande, RS, 96203-900, Brazil.
Programa de Pós Graduação em Ciências da Saúde, Universidade Federal do Rio Grande (FURG), Rua Visconde de Paranaguá 102 Centro, Rio Grande, RS, 96203-900, Brazil.
Environ Sci Pollut Res Int. 2017 Jul;24(20):16806-16814. doi: 10.1007/s11356-017-9312-y. Epub 2017 Jun 1.
The present study assessed the DNA damage in environmentally exposed volunteers living in seven municipalities in an industrial coal region, through the use of the comet assay with blood cells and the micronucleus test with buccal cells. Blood and buccal smears were collected from 320 male volunteers living in seven cities inserted in a coal region. They were ages of 18 and 50 years and also completed a questionnaire intended to identify factors associated with DNA damage through a Poisson regression analysis. The comet assay detected significant differences in DNA damage in volunteers from different municipalities, and neighboring cities (Pedras Altas, Aceguá, and Hulha Negra) had a higher level of DNA damage in relation to control city. Some of the risk factors associated with identified DNA lesions included residence time and life habits. On the other hand, the micronucleus test did not identify differences between the cities studied, but the regression analysis identified risk factors such as age and life habits (consumption of mate tea and low carbohydrates diet). We conclude that there are differences in the DNA damage of volunteers from different cities of the carboniferous region, but the presence of micronuclei in the oral mucosa does not differ between the same cities. Furthermore, we alert that some related factors may increase the risk of genotoxicity, such as residence location and time, and living and food habits. Finally, we suggest the need for continuous biomonitoring of the population, as well as for investing in health promotion in these vulnerable populations.
本研究通过对血细胞进行彗星试验和对颊细胞进行微核试验，评估了生活在工业煤炭产区七个市的环境暴露志愿者的DNA损伤情况。从生活在煤炭产区七个城市的320名男性志愿者身上采集了血液和颊部涂片。他们的年龄在18至50岁之间，并且还完成了一份问卷，旨在通过泊松回归分析确定与DNA损伤相关的因素。彗星试验检测到不同市的志愿者在DNA损伤方面存在显著差异，与对照市相比，相邻城市（佩德拉斯阿尔塔斯、阿塞瓜和乌尔哈内格拉）的DNA损伤水平更高。与已确定的DNA损伤相关的一些风险因素包括居住时间和生活习惯。另一方面，微核试验未发现所研究城市之间存在差异，但回归分析确定了年龄和生活习惯（饮用马黛茶和低碳水化合物饮食）等风险因素。我们得出结论，石炭纪地区不同城市的志愿者在DNA损伤方面存在差异，但同一城市口腔黏膜中的微核存在情况并无不同。此外，我们提醒，一些相关因素可能会增加遗传毒性风险，如居住地点和时间以及生活和饮食习惯。最后，我们建议需要对人群进行持续的生物监测，并在这些弱势群体中开展健康促进工作。
