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Arg i 系统：精氨酸丰富的穿透肽标记蛋白的一步纯化。

The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides.

机构信息

Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.

Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.

出版信息

Sci Rep. 2017 May 25;7(1):2619. doi: 10.1038/s41598-017-02432-6.

DOI:10.1038/s41598-017-02432-6
PMID:28572575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5453957/
Abstract

The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs.

摘要

细胞穿透肽 (CPP) 的发现为将蛋白质递送到人体细胞开辟了新的前景。人们认为，在未来，CPP 介导的治疗性蛋白质的运输可能会在人类疾病的治疗中得到应用。尽管如此，到目前为止，还没有一种快速、简单的方法可以从 CPP 标记的蛋白质中纯化出不含其他标记的蛋白质。为了填补这一空白，我们开发了 Argi 系统，用于一步纯化富含精氨酸的 CPP 标记的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/35ba243f956a/41598_2017_2432_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/d312db02d2a9/41598_2017_2432_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/5b0f6edbd88c/41598_2017_2432_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/aacb72e77219/41598_2017_2432_Fig6_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/16dd1eefb4bb/41598_2017_2432_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/ef5f6103a7f9/41598_2017_2432_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/35ba243f956a/41598_2017_2432_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/153c16abe59f/41598_2017_2432_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/ac17a2f5f61e/41598_2017_2432_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/8521e9511243/41598_2017_2432_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/d312db02d2a9/41598_2017_2432_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/5b0f6edbd88c/41598_2017_2432_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/aacb72e77219/41598_2017_2432_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/dd2ffdf96938/41598_2017_2432_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/16dd1eefb4bb/41598_2017_2432_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/ef5f6103a7f9/41598_2017_2432_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67da/5453957/35ba243f956a/41598_2017_2432_Fig10_HTML.jpg

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