纯化革兰氏阳性菌细胞壁的制备及其在胎盘和胎儿组织中的检测
Preparation of Purified Gram-positive Bacterial Cell Wall and Detection in Placenta and Fetal Tissues.
作者信息
Mann Beth, Loh Lip Nam, Gao Geli, Tuomanen Elaine
机构信息
Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis TN, USA.
出版信息
Bio Protoc. 2016 Dec 5;6(23). doi: 10.21769/BioProtoc.2037.
Abstract
Cell wall is a complex biopolymer on the surface of all Gram-positive bacteria. During infection, cell wall is recognized by the innate immune receptor Toll-like receptor 2 causing intense inflammation and tissue damage. In animal models, cell wall traffics from the blood stream to many organs in the body, including brain, heart, placenta and fetus. This protocol describes how to prepare purified cell wall from , detect its distribution in animal tissues, and study the tissue response using the placenta and fetal brain as examples.
摘要
细胞壁是所有革兰氏阳性菌表面的一种复杂生物聚合物。在感染过程中，细胞壁被天然免疫受体Toll样受体2识别，引发强烈炎症和组织损伤。在动物模型中，细胞壁从血流进入身体的许多器官，包括脑、心脏、胎盘和胎儿。本方案描述了如何从[具体来源]制备纯化的细胞壁，检测其在动物组织中的分布，并以胎盘和胎儿脑为例研究组织反应。
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本文引用的文献
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