Nieto-Yañez O J, Resendiz-Albor A A, Ruiz-Hurtado P A, Rivera-Yañez N, Rodriguez-Canales M, Rodriguez-Sosa M, Juarez-Avelar I, Rodriguez-Lopez M G, Canales-Martinez M M, Rodriguez-Monroy M A
Laboratorio de Inmunidad de Mucosas. Sección de Estudios de Posgrado e Investigación. Escuela Superior de Medicina, del Instituto Politécnico Nacional. Av. Plan de San Luis S/N, Colonia Casco de Santo Tomas, Miguel Hidalgo, CP. 11350. Ciudad de México, D.F.
Lab. Inmunobiología (L-321). UNAM FES Iztacala. Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, Tlalnepantla, Estado de México, C.P. 54090.
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):188-197. doi: 10.21010/ajtcam.v14i2.20. eCollection 2017.
Cutaneous leishmaniasis lacks effective and well-tolerated treatments. The current therapies mainly rely on antimonial drugs that are inadequate because of their poor efficacy. Traditional medicine offers a complementary alternative for the treatment of various diseases. Additionally, several plants have shown success as anti-leishmanial agents. Therefore, we sought to evaluate the and activity of MEBA against .
Methanolic extract of was obtained by macetration, after we determined anti-leishmanial activity of MEBA by MTT assay and the induced apoptosis in promastigotes by flow cytometry. To analyze the anti-leishmanial activity, we used infected mice that were treated and not treated with MEBA and we determined the levels of cytokines using ELISA. The phytochemical properties were determined by CG-MS and DPPH assay.
We determined of LC of 0.408 mg/mL of MEBA for anti-leishmanial activity. MEBA induced apoptosis in promastigotes (15.3% ± 0.86). Treated mice exhibited smaller lesions and contained significantly fewer parasites than did untreated mice; in addition, we found that IFN-γ and TNF-α increased in the sera of MEBA-treated mice. GC-MS analysis showed that podophyllotoxin was the most abundant compound. Evaluation of the activity by DPPH assay demonstrated an SC of 11.72 μg/mL.
Based on the above data, it was concluded that MEBA is a good candidate in the search for new anti-leishmanial agents.
皮肤利什曼病缺乏有效且耐受性良好的治疗方法。目前的治疗主要依赖于锑剂，但由于其疗效不佳而存在不足。传统医学为各种疾病的治疗提供了一种补充选择。此外，几种植物已显示出作为抗利什曼原虫剂的成效。因此，我们试图评估MEBA对[具体对象未明确]的[具体作用未明确]和活性。
通过浸渍法获得[具体植物未明确]的甲醇提取物，之后我们通过MTT法测定MEBA的抗利什曼原虫活性，并通过流式细胞术检测前鞭毛体中的诱导凋亡情况。为分析MEBA的抗利什曼原虫活性，我们使用了经MEBA处理和未处理的感染小鼠，并通过酶联免疫吸附测定法测定细胞因子水平。通过气相色谱 - 质谱联用仪（CG-MS）和二苯基苦味酰基自由基（DPPH）测定法确定植物化学性质。
我们确定MEBA对[具体对象未明确]的抗利什曼原虫活性的半数致死浓度（LC）为0.408 mg/mL。MEBA诱导前鞭毛体凋亡（15.3% ± 0.86）。与未处理的小鼠相比，经处理的小鼠病变较小且寄生虫数量明显更少；此外，我们发现MEBA处理的小鼠血清中γ-干扰素（IFN-γ）和肿瘤坏死因子-α（TNF-α）增加。气相色谱 - 质谱联用仪（GC-MS）分析表明鬼臼毒素是最丰富的化合物。通过DPPH测定法评估活性显示半数抑制浓度（SC）为11.72 μg/mL。
基于上述数据，得出结论认为MEBA是寻找新型抗利什曼原虫剂的良好候选物。
