Meng Xiaofeng, Shi Baozhong
Department of Neurosurgery, First Affiliated Hospital, Henan University of Science & Technology, No. 36 Tongji Street, Luonan New District, Luoyang, 471003, Henan, People's Republic of China.
Biotechnol Lett. 2017 Sep;39(9):1351-1358. doi: 10.1007/s10529-017-2373-7. Epub 2017 Jun 1.
To investigate the roles of miR-215 in high-grade glioma and to clarify the regulation of retinoblastoma 1 (RB1) by miR-215.
miR-215 is frequently up-regulated in high-grade glioma tissues. Increased miR-215 expression is significantly associated with World Health Organization grade (P < 0.01) tumor size (P < 0.05) and poor prognosis (P < 0.01). Over-expression of miR-215 promoted cell proliferation and knockdown of miR-215 inhibited cell proliferation in vitro. RB1 was identified as a direct and functional target of miR-215. RB1 is generally down-regulated in glioma tissues and its expression inversely correlated with miR-215, which is up-regulated in high-grade glioma tissues, and its expression was negatively correlated with miR-215.
The new miR-215/RB1 axis provides new insights into the molecular mechanism and treatment for glioma.
研究miR-215在高级别胶质瘤中的作用，并阐明miR-215对视网膜母细胞瘤1（RB1）的调控作用。
miR-215在高级别胶质瘤组织中经常上调。miR-215表达增加与世界卫生组织分级（P < 0.01）、肿瘤大小（P < 0.05）及预后不良（P < 0.01）显著相关。miR-215过表达促进细胞增殖，而敲低miR-215则在体外抑制细胞增殖。RB1被确定为miR-215的直接功能性靶点。RB1在胶质瘤组织中通常下调，其表达与miR-215呈负相关，miR-215在高级别胶质瘤组织中上调。
新的miR-215/RB1轴为胶质瘤的分子机制和治疗提供了新的见解。
