Affiliated Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, Heilongjiang, China (mainland).
Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China (mainland).
Med Sci Monit. 2018 Oct 7;24:7137-7145. doi: 10.12659/MSM.910295.
BACKGROUND Accumulating studies demonstrate that microRNAs play crucial roles in multiple processes of cancer progression. Lower levels of miR-183 have been observed in diverse types of tumors but the mechanism and precise function of miR-183-5p in cervical cancer have largely not been investigated. MATERIAL AND METHODS The level of miR-183-5p in different cervical cancer cell lines and clinical tissues was detected qRT-PCR assays. Transwell and wound-healing migration assays were conducted to assess the functional roles of miR-183-5p in over-expressing cervical cancer cells in vitro. Rescue assays were carried out to confirm the contribution of integrin subunit Beta 1 (ITGB1) to the aggressiveness of cancer cells regulated by miR-183-5p. RESULTS miR-183-5p was reduced in clinical tissues of cervical cancer and cell lines when compared to the normal subjects and normal cervical epithelial cell line, respectively. In addition, over-expression of miR-183-5p markedly inhibited migration and invasion in cervical cancer cells, and increased aggressiveness was observed in miR-183-5p inhibitor transfected cells. Furthermore, the luciferase reporter assays revealed that ITGB1 was the gene directly regulated by miR-183-5p. Notably, a negative association between the ITGB1 and miR-183-5p was found, and the gene expressions of ITGB1 was mediated by miR-183-5p in cervical cancer cells. CONCLUSIONS miR-183-5p serves as a latent anti-oncogene by targeting the metastasis-promoter gene, ITGB1.
越来越多的研究表明，microRNAs 在癌症进展的多个过程中发挥着关键作用。在不同类型的肿瘤中观察到 miR-183 的水平降低，但 miR-183-5p 在宫颈癌中的机制和精确功能在很大程度上尚未得到研究。
通过 qRT-PCR 检测不同宫颈癌细胞系和临床组织中 miR-183-5p 的水平。进行 Transwell 和划痕愈合迁移实验，以评估 miR-183-5p 在体外过表达宫颈癌细胞中的功能作用。进行挽救实验，以确认整合素亚基β 1（ITGB1）对 miR-183-5p 调节的癌细胞侵袭性的贡献。
与正常个体和正常宫颈上皮细胞系相比，miR-183-5p 在宫颈癌临床组织和细胞系中降低。此外，miR-183-5p 的过表达显著抑制了宫颈癌细胞的迁移和侵袭，并且在转染 miR-183-5p 抑制剂的细胞中观察到侵袭性增加。此外，荧光素酶报告基因实验表明，ITGB1 是受 miR-183-5p 直接调控的基因。值得注意的是，发现 ITGB1 和 miR-183-5p 之间存在负相关，并且 ITGB1 的基因表达受宫颈癌细胞中 miR-183-5p 的介导。
miR-183-5p 通过靶向转移促进基因 ITGB1 作为潜在的抑癌基因。
