微电镜直接电子探测器的电子计数。
Electron counting with direct electron detectors in MicroED.
机构信息
Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Physiology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
Structure. 2023 Dec 7;31(12):1504-1509.e1. doi: 10.1016/j.str.2023.10.011. Epub 2023 Nov 21.
The combination of high sensitivity and rapid readout makes it possible for electron-counting detectors to record cryogenic electron microscopy data faster and more accurately without increasing the number of electrons used for data collection. This is especially useful for MicroED of macromolecular crystals where the strength of the diffracted signal at high resolution is comparable to the surrounding background. The ability to decrease fluence also alleviates concerns about radiation damage which limits the information that can be recovered from a diffraction measurement. The major concern with electron-counting direct detectors lies at the low end of the resolution spectrum: their limited linear range makes strong low-resolution reflections susceptible to coincidence loss and careful data collection is required to avoid compromising data quality. Nevertheless, these cameras are increasingly deployed in cryo-EM facilities, and several have been successfully used for MicroED. Provided coincidence loss can be minimized, electron-counting detectors bring high potential rewards.
高灵敏度和快速读出的组合使得电子计数探测器能够更快、更准确地记录低温电子显微镜数据，而无需增加用于数据收集的电子数量。这对于大分子晶体的微电子衍射（MicroED）特别有用，因为在高分辨率下，衍射信号的强度与周围背景相当。降低通量的能力也减轻了人们对辐射损伤的担忧，辐射损伤限制了可以从衍射测量中恢复的信息量。电子计数直接探测器的主要问题在于分辨率谱的低端：它们有限的线性范围使得强低分辨率反射容易受到符合损失的影响，因此需要仔细采集数据以避免影响数据质量。然而，这些相机在低温电子显微镜设施中越来越多地得到部署，并且已经成功地用于 MicroED。只要能够将符合损失降到最低，电子计数探测器就具有很高的潜在回报。
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