Schmidt Marius
Kenwood Interdisciplinary Research Complex, Physics Department, University of Wisconsin-Milwaukee, Room 3087, 3135 North Maryland Avenue, Milwaukee, WI, 53211, USA.
Methods Mol Biol. 2017;1607:273-294. doi: 10.1007/978-1-4939-7000-1_11.
Time-resolved macromolecular crystallography unifies protein structure determination with chemical kinetics. With the advent of fourth generation X-ray sources the time-resolution can be on the order of 10-40 fs, which opens the ultrafast time scale to structure determination. Fundamental motions and transitions associated with chemical reactions in proteins can now be observed. Moreover, new experimental approaches at synchrotrons allow for the straightforward investigation of all kind of reactions in biological macromolecules. Here, recent developments in the field are reviewed.
时间分辨大分子晶体学将蛋白质结构测定与化学动力学结合起来。随着第四代X射线源的出现，时间分辨率可达10-40飞秒量级，这为结构测定开启了超快时间尺度。现在可以观察到与蛋白质化学反应相关的基本运动和转变。此外，同步加速器上的新实验方法使得对生物大分子中各种反应的直接研究成为可能。在此，对该领域的最新进展进行综述。
