Fives-Taylor P M, Thompson D W
Infect Immun. 1985 Mar;47(3):752-9. doi: 10.1128/iai.47.3.752-759.1985.
Seventeen mutants of Streptococcus sanguis FW213 nonadherent to saliva-coated spheroidal hydroxyapatite were isolated after mutagenesis with ethyl methanesulfonate, nitrosoguanidine, nitrous acid, hydroxylamine, or 2-aminopurine. Enrichment for nonadherent mutants was accomplished by successive adsorptions of the adherent strains to saliva-coated hydroxyapatite. After enrichment, variant colonial morphology on tryptic agar was used as a screening technique for selection of nonadherent mutants, with loss of colonial opacity frequently associated with loss of adherence ability. These mutants were further characterized for additional surface properties, including twitching motility, saliva-induced aggregation, coaggregation with Actinomyces species, surface hydrophobicity, and presence of fimbriae. Results from these assays indicated that the nonadherent mutants fell into six phenotypic groups. A correlation between the loss of adherence ability, a decrease in cell fimbriation, and a decrease in surface hydrophobicity is apparent.
用甲磺酸乙酯、亚硝基胍、亚硝酸、羟胺或2-氨基嘌呤诱变后，分离出17株不粘附于唾液包被的球形羟基磷灰石的血链球菌FW213突变体。通过将粘附菌株连续吸附到唾液包被的羟基磷灰石上来富集非粘附突变体。富集后，用胰蛋白酶琼脂上的变异菌落形态作为筛选技术来选择非粘附突变体，菌落透明度的丧失通常与粘附能力的丧失有关。对这些突变体的其他表面特性进行了进一步表征，包括颤动运动性、唾液诱导的聚集、与放线菌属的共聚、表面疏水性和菌毛的存在。这些试验的结果表明，非粘附突变体分为六个表型组。粘附能力的丧失、细胞菌毛形成的减少和表面疏水性的降低之间的相关性是明显的。
