Liljemark W F, Bloomquist C G, Germaine G R
Infect Immun. 1981 Mar;31(3):935-41. doi: 10.1128/iai.31.3.935-941.1981.
Several in vitro assay systems to measure the adherence of human dental plaque bacteria to solid surfaces such as teeth, glass, and hydroxyapatite have been published. In many studies a variety of macromolecular solutes have been used to study the adherence process. Often these solutes are able to aggregate the test bacterial and thus may alter the outcome of adherence experiments. In this study, the effects of the aggregation of Streptococcus sanguis on adherence to spheroidal hydroxyapatite is described. Adherence of preformed aggregates and of bacteria which were aggregating during the adherence reaction was examined. Bacteria were aggregated with whole saliva, concanavalin A, and wheat germ lectin. Further effects of the coaggregation of S. mitis and Actinomyces viscosus to saliva-coated spheroidal hydroxyapatite are presented. These studies suggest that formation of large aggregates resulted in a decrease in the numbers of organisms which adhered. In contrast, the formation of small aggregates actually increased the numbers of bacteria that adhered. All increases in adherent bacteria occurred at low concentrations of aggregating substance in which visible bacterial aggregation was not evident. The data indicate that adequate dose-response experiments must be performed to ensure that solutes used as probes to study adherence mechanisms do not affect the adherence simply as a result of aggregation of the test microorganisms.
已有数种体外检测系统用于测定人类牙菌斑细菌对牙齿、玻璃和羟基磷灰石等固体表面的黏附，相关研究已发表。在许多研究中，多种大分子溶质被用于研究黏附过程。这些溶质常常能够使受试细菌聚集，从而可能改变黏附实验的结果。本研究描述了血链球菌聚集对其黏附球形羟基磷灰石的影响。研究了预先形成的聚集体以及在黏附反应过程中正在聚集的细菌的黏附情况。细菌与全唾液、伴刀豆球蛋白A和麦胚凝集素发生聚集。还介绍了缓症链球菌与黏性放线菌共聚集对唾液包被的球形羟基磷灰石的进一步影响。这些研究表明，大聚集体的形成导致黏附的微生物数量减少。相反，小聚集体的形成实际上增加了黏附细菌的数量。黏附细菌数量的所有增加都发生在聚集物质浓度较低的情况下，此时肉眼可见的细菌聚集并不明显。数据表明，必须进行充分的剂量反应实验，以确保用作研究黏附机制探针的溶质不会仅仅由于受试微生物的聚集而影响黏附。
