Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Chemistry and Material Sciences, Heilongjiang University, Harbin, Heilongjiang 150080, China.
Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, Heilongjiang 150080, China; Laboratory for Reproductive Health, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen, Guangdong 518055, China.
Carbohydr Polym. 2017 Sep 1;171:267-280. doi: 10.1016/j.carbpol.2017.05.022. Epub 2017 May 10.
We have synthesized N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan (N-2-HFCC) with higher water solubility than chitosan. To evaluate its potential as delivery carrier, NDV encapsulated in N-2-HFCC/CMC nanoparticles (NDV/La Sota-N-2-HFCC/CMC-NPs) with a diameter of 252.2±32.68nm, Zeta potential of 41.1±0.89mV, encapsulation efficiency of 96.6±0.72% and loading capacity of 53.2±1.11% were prepared. NDV/La Sota-N-2-HFCC/CMC-NPs are a novel type of attenuated live vaccine encapsulated in nanoparticles. These nanoparticles displayed lower cytotoxicity and higher stability. Their bioactivity was maintained when they were stored for three months at the room temperature. Release assay in vitro showed that NDV could be sustainably released after an initial burst release. In vivo immunization showed that immunization of specific pathogen free chickens with NDV/La Sota-N-2-HFCC/CMC-NPs intranasally induced high titers of serum antibody, significantly promoted lymphocyte proliferation and caused higher levels of interleukine-2 and interference-γ. These results indicated that N-2-HFCC/CMC nanoparticles could serve as an efficient and safe delivery vehicle for mucosal immunity, and have a great potential in medical applications.
我们合成了 N-2-羟丙基二甲乙基氯化铵壳聚糖（N-2-HFCC），其水溶性高于壳聚糖。为了评估其作为递送载体的潜力，我们将 NDV 包裹在 N-2-HFCC/CMC 纳米颗粒（NDV/La Sota-N-2-HFCC/CMC-NPs）中，该纳米颗粒的直径为 252.2±32.68nm，Zeta 电位为 41.1±0.89mV，包封效率为 96.6±0.72%，载药量为 53.2±1.11%。NDV/La Sota-N-2-HFCC/CMC-NPs 是一种新型的包裹在纳米颗粒中的减毒活疫苗。这些纳米颗粒显示出较低的细胞毒性和更高的稳定性。当它们在室温下储存三个月时，其生物活性得以保持。体外释放试验表明，NDV 可以在初始突释后持续释放。体内免疫试验表明，NDV/La Sota-N-2-HFCC/CMC-NPs 经鼻腔免疫 SPF 鸡可诱导高滴度的血清抗体，显著促进淋巴细胞增殖，并引起更高水平的白细胞介素-2 和干扰素-γ。这些结果表明，N-2-HFCC/CMC 纳米颗粒可以作为一种高效、安全的黏膜免疫递药系统，在医学应用中有很大的潜力。
