Miao Mingsan, Yan Xiaoli, Guo Lin, Shao Shuai
Departments of Science and Technology, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.
Saudi Pharm J. 2017 May;25(4):607-614. doi: 10.1016/j.jsps.2017.04.032. Epub 2017 Apr 28.
The effect of the Rabdosia rubescens total flavonoids on focal cerebral ischemia reperfusion model in rats was observed. The model group, nimodipine group, cerebral collateral group, and large, medium and small dose group of the Rabdosia rubescens total flavonoids were administered with corresponding drugs but sham operation group and model group were administered the same volume of 0.5%CMC, 1 times a day, continuous administration of 7 d. After 1 h at 7 d to medicine, left incision in the middle of the neck of rats after anesthesia, we can firstly expose and isolate the left common carotid artery (CCA), and then expose external carotid artery (ECA) and internal carotid artery (ICA). The common carotid artery and the external carotid artery are ligated. Then internal carotid artery with arterial clamp is temporarily clipped. Besides, cut the incision of 0.2 mm from 5 cm of the bifurcation of the common carotid artery. A thread Line bolt is inserted with more than 18-20 mm from bifurcation of CCA into the internal carotid artery until there is resistance. Then the entrance of the middle cerebral artery is blocked and internal carotid artery is ligated (the blank group only exposed the left blood vessel without Plugging wire). Finally it is gently pulled out the plug line after 2 h. : Compared with the model mice, Rabdosia rubescens total flavonoids can significantly relieve the injury of brain in hippocampus and cortex nerve cells; experimental rat focal cerebral ischemia was to improve again perfusion model of nerve function defect score mortality; significantly reduce brain homogenate NOS activity and no content, MDA, IL-1, TNF-a, ICAM-1 content; increase in brain homogenate SOD and ATPase activity ( < 0.05, < 0.01); and reduce the serum S-100β protein content. Each dose group of the Rabdosia rubescens total flavonoids has a better Improvement effect on focal cerebral ischemia reperfusion model in rats.
观察冬凌草总黄酮对大鼠局灶性脑缺血再灌注模型的影响。模型组、尼莫地平组、脑络通组以及冬凌草总黄酮大、中、小剂量组给予相应药物，假手术组和模型组给予等体积的0.5%羧甲基纤维素钠，每天1次，连续给药7 d。给药7 d后1 h，麻醉大鼠后在颈部正中做切口，先暴露并分离左侧颈总动脉（CCA），再暴露颈外动脉（ECA）和颈内动脉（ICA）。结扎颈总动脉和颈外动脉。然后用动脉夹暂时夹闭颈内动脉。此外，在颈总动脉分叉处5 cm处做0.2 mm的切口。将线栓从颈总动脉分叉处插入颈内动脉18 - 20 mm以上直至有阻力。然后阻断大脑中动脉入口并结扎颈内动脉（空白组仅暴露左侧血管不插线栓）。最后2 h后轻轻拔出栓线。结果显示：与模型小鼠相比，冬凌草总黄酮能显著减轻海马和皮质神经细胞的脑损伤；改善实验大鼠局灶性脑缺血再灌注模型的神经功能缺损评分及死亡率；显著降低脑匀浆中一氧化氮合酶（NOS）活性、一氧化氮（NO）含量、丙二醛（MDA）、白细胞介素 - 1（IL - 1）、肿瘤坏死因子 - α（TNF - α）、细胞间黏附分子 - 1（ICAM - 1）含量；提高脑匀浆超氧化物歧化酶（SOD）和三磷酸腺苷酶（ATPase）活性（P < 0.05，P < 0.01）；降低血清S - 100β蛋白含量。冬凌草总黄酮各剂量组对大鼠局灶性脑缺血再灌注模型均有较好的改善作用。
