Zhao Junxing, Jin Yaqian, Du Min, Liu Wenzhong, Ren Youshe, Zhang Chunxiang, Zhang Jianxin
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, PR China.
Department of Animal Science, Washington State University, Pullman, 99164, United States.
Theriogenology. 2017 Jul 15;97:50-56. doi: 10.1016/j.theriogenology.2017.04.010. Epub 2017 Apr 5.
Wine grape pomace (WGP) contains a rich source of polyphenols that can act as powerful antioxidants. The objective of this study was to investigate the effect of dietary WGP supplementation on antioxidative activity and epididymal sperm quality in rams. The rams were raised either under free-range or pen conditions, and the pen-raised rams were fed a WGP-containing diet (0, 5% and 10% of dry matter basis) for 74 days. An increase in the concentrations of reactive oxygen species (ROS, P < 0.05) and malondialdehyde (MDA, P < 0.05) were observed in the testes of rams subjected to restraint stress, and dietary WGP supplementation effectively decreased their contents (P < 0.05). Restraint stress reduced both weight and volume of testes, and impaired sperm quality. Dietary WGP supplementation increased testes weight, sperm concentration, motility and acrosomal integrity, and decreased sperm deformity in pen-raised animals (P < 0.05). The total antioxidative capacity (T-AOC) and catalase activity were decreased in the testes of pen-raised lambs (P < 0.05), and T-AOC, catalase, glutathione peroxidase 4 (GPx4) and superoxide dismutase (SOD) activity were increased when rams were fed the WGP-containing diet (P < 0.05). With the exception of SOD and GPx4, the mRNA contents of catalase and nuclear factor-like-2 factor (Nrf2) did not vary among the groups, and greater protein levels of SOD, catalase and GPx4 were observed in WGP-treated lambs (P < 0.05). Taken together, these results suggest that WGP can be used as a feed ingredient in rams to alleviate restraint induced oxidative stress and improve epididymal sperm quality.
酿酒葡萄果渣（WGP）富含多酚类物质，这些多酚可作为强大的抗氧化剂。本研究的目的是调查日粮中添加WGP对公羊抗氧化活性和附睾精子质量的影响。公羊分别在自由放养或围栏饲养条件下饲养，对围栏饲养的公羊饲喂含WGP的日粮（干物质基础的0%、5%和10%），持续74天。在遭受束缚应激的公羊睾丸中，活性氧（ROS，P<0.05）和丙二醛（MDA，P<0.05）浓度增加，而日粮中添加WGP可有效降低它们的含量（P<0.05）。束缚应激降低了睾丸的重量和体积，并损害了精子质量。日粮中添加WGP增加了围栏饲养动物的睾丸重量、精子浓度、活力和顶体完整性，并降低了精子畸形率（P<0.05）。围栏饲养羔羊的睾丸中总抗氧化能力（T-AOC）和过氧化氢酶活性降低（P<0.05），当公羊饲喂含WGP的日粮时，T-AOC、过氧化氢酶、谷胱甘肽过氧化物酶4（GPx4）和超氧化物歧化酶（SOD）活性增加（P<0.05）。除SOD和GPx4外，各处理组间过氧化氢酶和核因子样-2因子（Nrf2）的mRNA含量没有差异，但在WGP处理的羔羊中观察到SOD、过氧化氢酶和GPx4的蛋白质水平更高（P<0.05）。综上所述，这些结果表明WGP可作为公羊的饲料成分，以减轻束缚诱导的氧化应激并改善附睾精子质量。
