College of Life Sciences, Sichuan University , Chengdu 610064, China.
School of Life Science and Technology, ShanghaiTech University , Shanghai 201210, China.
ACS Appl Mater Interfaces. 2017 Jun 28;9(25):21200-21208. doi: 10.1021/acsami.7b05662. Epub 2017 Jun 13.
Semiconducting polymer dots (Pdots) have shown great promise in biomedical applications, including biosensing, drug delivery, and live imaging of cells and biomolecules. Insight into the mechanism and regulation of cellular uptake and intracellular metabolism of Pdots is important for the development of superior Pdots-based theranostic nanoconjugates. Herein, we performed real-time imaging of endocytosis and intracellular trafficking of a type of fluorescent Pdots that showed excellent biocompatibility in various types of cells. The endocytic routes and kinetics of Pdots were differently regulated in distinct cell types. Following endocytosis, Pdots were transported in vesicles along microtubule and destined for lysosomes. Furthermore, our results revealed exosome-mediated extracellular release of Pdots and have tracked the dynamic process at the single particle level. These results provide new insight into the design of more effective and selective imaging probes as well as drug carriers.
半导体聚合物点（Pdots）在生物医学应用中显示出巨大的潜力，包括生物传感、药物输送和细胞及生物分子的活体成像。深入了解 Pdots 的细胞摄取和细胞内代谢的机制和调控对于开发基于 Pdots 的优越治疗性纳米缀合物非常重要。在此，我们对一类荧光 Pdots 的内吞作用和细胞内转运进行了实时成像，该 Pdots 在各种类型的细胞中表现出优异的生物相容性。Pdots 的内吞途径和动力学在不同的细胞类型中受到不同的调节。内吞作用后，Pdots 沿着微管被转运到溶酶体。此外，我们的结果揭示了 Pdots 经外泌体介导的细胞外释放，并在单颗粒水平上跟踪了动态过程。这些结果为更有效和选择性的成像探针以及药物载体的设计提供了新的见解。
