College of Life Sciences, Sichuan University, Chengdu, China.
Division of Physical Biology and Bioimaging Center, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
Cell Prolif. 2019 Mar;52(2):e12556. doi: 10.1111/cpr.12556. Epub 2019 Feb 1.
Nanocarriers can greatly enhance the cellular uptake of therapeutic agents to regulate cell proliferation and metabolism. Nevertheless, further application of nanocarriers is often limited by insufficient understanding of the mechanisms of their uptake and intracellular behaviour.
Fluorescent polymer dots (Pdots) are coated with synthetic octaarginine peptides (R8) and are analysed for cellular uptake and intracellular transportation in HeLa cervical cancer cells via single particle tracking.
Surface modification with the R8 peptide efficiently improves both cellular uptake and endosomal escape of Pdots. With single particle tracking, we capture the dynamic process of internalization and intracellular trafficking of R8-Pdots, providing new insights into the mechanism of R8 in facilitating nanostructure-based cellular delivery. Furthermore, our results reveal R8-Pdots as a novel type of autophagy inducer.
This study provides new insights into R8-mediated cellular uptake and endosomal escape of nanocarriers. It potentiates biological applications of Pdots in targeted cell imaging, drug delivery and gene regulation.
纳米载体可以极大地提高治疗剂的细胞摄取率，从而调节细胞增殖和代谢。然而，纳米载体的进一步应用通常受到对其摄取和细胞内行为机制的理解不足的限制。
荧光聚合物点（Pdots）用合成的八精氨酸肽（R8）进行涂层，并通过单颗粒跟踪分析其在 HeLa 宫颈癌细胞中的细胞摄取和细胞内运输情况。
用 R8 肽进行表面修饰可以有效地提高 Pdots 的细胞摄取率和内涵体逃逸率。通过单颗粒跟踪，我们捕捉到 R8-Pdots 内化和细胞内运输的动态过程，为 R8 促进基于纳米结构的细胞递药机制提供了新的见解。此外，我们的结果揭示了 R8-Pdots 是一种新型的自噬诱导剂。
本研究为 R8 介导的纳米载体的细胞摄取和内涵体逃逸提供了新的见解。它增强了 Pdots 在靶向细胞成像、药物输送和基因调控中的生物应用。
