Dikina A D, Lai B P, Cao M, Zborowski M, Alsberg E
Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106, USA.
Biomater Sci. 2017 Jun 27;5(7):1241-1245. doi: 10.1039/c7bm00061h.
Using a novel magnetic field bioreactor, this work evaluated the chondrogenesis of scaffold-free human mesenchymal stem cell sheets in response to static and variable magnetic fields, as well as mechanical stimulation via 4.4 μm magnetic particles. Neither static nor variable magnetic fields generated by 1.44-1.45 T permanent magnets affected cartilage formation. Notably, magnetic field-induced mechanical stimulation by magnetic particles, which applied forces to the cells and ECM statically (4.39 pN) or cyclically (1.06-63.6 pN; 16.7 mHz), also did not affect cartilage formation.
利用一种新型磁场生物反应器，本研究评估了无支架人骨髓间充质干细胞片在静态和可变磁场以及通过4.4μm磁性颗粒进行机械刺激下的软骨生成情况。由1.44 - 1.45T永磁体产生的静态或可变磁场均未影响软骨形成。值得注意的是，磁性颗粒引起的磁场诱导机械刺激，无论是静态（4.39 pN）还是周期性（1.06 - 63.6 pN；16.7 mHz）对细胞和细胞外基质施加力，也未影响软骨形成。
