Muszbek L, Adány R, Szegedi G, Polgár J, Kávai M
Thromb Res. 1985 Feb 1;37(3):401-10. doi: 10.1016/0049-3848(85)90069-6.
The presence of Factor XIII subunit a was demonstrated in human monocytes by immunoperoxidase staining using specific antisera against Factor XIII and its subunits. This finding was verified by immunobiochemical techniques, as well. In an immunoblotting system after SDS polyacrylamide gel electrophoresis of denatured monocyte homogenate a protein band comigrating with Factor XIII subunit a showed positive reaction with antibodies against this subunit or whole Factor XIII. In contrast, no subunit b of Factor XIII could be detected by either of these methods in monocytes. Activity measurements were carried out by the dansylcadaverine incorporation assay in the absence and presence of anti-Factor XIII antibody with and without thrombin activation. The expression of transglutaminase activity required thrombin and was completely abolished in presence of anti- Factor XIII antibody, which clearly indicate that practically all the transglutaminase activity measured in monocytes comes from Factor XIII. Factor XIII of monocytes and macrophages might have a role in formation of focal fibrin thrombi as well as in organization of stable, fibrinolysis resistant fibrin clot at the site of inflammation or around tumor cells.
使用针对因子 XIII 及其亚基的特异性抗血清，通过免疫过氧化物酶染色在人单核细胞中证实了因子 XIII 亚基 a 的存在。这一发现也通过免疫生化技术得到了验证。在对变性单核细胞匀浆进行 SDS 聚丙烯酰胺凝胶电泳后的免疫印迹系统中，与因子 XIII 亚基 a 共迁移的一条蛋白带与针对该亚基或完整因子 XIII 的抗体呈现阳性反应。相比之下，通过这两种方法在单核细胞中均未检测到因子 XIII 的亚基 b。在有无抗因子 XIII 抗体以及有无凝血酶激活的情况下，通过丹磺酰尸胺掺入法进行活性测量。转谷氨酰胺酶活性的表达需要凝血酶，并且在抗因子 XIII 抗体存在的情况下完全被消除，这清楚地表明在单核细胞中测得的几乎所有转谷氨酰胺酶活性都来自因子 XIII。单核细胞和巨噬细胞中的因子 XIII 可能在局部纤维蛋白血栓的形成以及在炎症部位或肿瘤细胞周围稳定的、抗纤维蛋白溶解的纤维蛋白凝块的组织化过程中发挥作用。
