Bennet Devasier, Viswanath Buddolla, Kim Sanghyo, An Jeong Ho
Department of Bionanotechnology, Gachon University, Seongnam, Gyeonggi-Do 461-701, Republic of Korea.
Department of Polymer Science & Engineering, Sungkyunkwan University, Suwon, Gyeonggi-Do 440-146, Republic of Korea.
Oncotarget. 2017 Jul 18;8(29):47861-47875. doi: 10.18632/oncotarget.18136.
The aim of this study was to analyze photo-dynamic and photo-pathology changes of different color light radiations on human adult skin cells. We used a real-time biophysical and biomechanics monitoring system for light-induced cellular changes in an in vitro model to find mechanisms of the initial and continuous degenerative process. Cells were exposed to intermittent, mild and intense (1-180 min) light with On/Off cycles, using blue, green, red and white light. Cellular ultra-structural changes, damages, and ECM impair function were evaluated by up/down-regulation of biophysical, biomechanical and biochemical properties. All cells exposed to different color light radiation showed significant changes in a time-dependent manner. Particularly, cell growth, stiffness, roughness, cytoskeletal integrity and ECM proteins of the human dermal fibroblasts-adult (HDF-a) cells showed highest alteration, followed by human epidermal keratinocytes-adult (HEK-a) cells and human epidermal melanocytes-adult (HEM-a) cells. Such changes might impede the normal cellular functions. Overall, the obtained results identify a new insight that may contribute to premature aging, and causes it to look aged in younger people. Moreover, these results advance our understanding of the different color light-induced degenerative process and help the development of new therapeutic strategies.
本研究的目的是分析不同颜色光辐射对成人皮肤细胞的光动力学和光病理学变化。我们使用实时生物物理和生物力学监测系统，在体外模型中观察光诱导的细胞变化，以探寻初始和持续退化过程的机制。细胞暴露于具有开/关循环的间歇性、轻度和强烈（1 - 180分钟）的蓝光、绿光、红光和白光下。通过生物物理、生物力学和生化特性的上调/下调来评估细胞超微结构变化、损伤和细胞外基质功能受损情况。所有暴露于不同颜色光辐射的细胞均呈现出随时间变化的显著变化。特别是，成人真皮成纤维细胞（HDF - a）的细胞生长、硬度、粗糙度、细胞骨架完整性和细胞外基质蛋白变化最为显著，其次是成人表皮角质形成细胞（HEK - a）和成人表皮黑素细胞（HEM - a）。这些变化可能会阻碍细胞的正常功能。总体而言，所获得的结果揭示了一个新的见解，即这可能导致过早衰老，并使年轻人看起来显老。此外，这些结果增进了我们对不同颜色光诱导的退化过程的理解，并有助于开发新的治疗策略。
