重组纤维调节素和核心蛋白聚糖对4T1乳腺癌细胞系中NF-κB和TGFβ1的影响。
Recombinant fibromodulin and decorin effects on NF-κB and TGFβ1 in the 4T1 breast cancer cell line.
作者信息
Dawoody Nejad Ladan, Biglari Alireza, Annese Tiziana, Ribatti Domenico
机构信息
Department of Molecular Medicine and Biochemistry Institute, University of Bern, 3012 Bern, Switzerland.
Department of Molecular Medicine and Genetics, Zanjan University of Medical Sciences, 45154 Zanjan, Iran.
出版信息
Oncol Lett. 2017 Jun;13(6):4475-4480. doi: 10.3892/ol.2017.5960. Epub 2017 Mar 31.
Abstract
Constitutive activation of nuclear factor-κB (NF-κB) stimulates cell proliferation and metastasis, and inhibits apoptosis in breast cancer. Transforming growth factor-β (TGF-β) signaling pathway is deregulated in breast cancer progression and metastasis. The aim of the present study was to investigate the inhibitory effects of the two small leucine rich proteoglycans fibromodulin (Fmod) and decorin (Dcn), overexpressed using adenovirus gene transfer, on NF-κB-activity and TGF-β1-expression in the highly metastatic 4T1 breast cancer cell line. The results demonstrate that Fmod and Dcn overexpression is associated with NF-κB and TGF-β1 downregulation, and that Fmod promotes this effect more effectively compared with Dcn.
摘要
核因子-κB（NF-κB）的组成性激活会刺激乳腺癌细胞的增殖和转移，并抑制其凋亡。转化生长因子-β（TGF-β）信号通路在乳腺癌进展和转移过程中失调。本研究旨在探讨通过腺病毒基因转移过表达的两种富含亮氨酸的小分子蛋白聚糖——纤调蛋白（Fmod）和核心蛋白聚糖（Dcn），对高转移性4T1乳腺癌细胞系中NF-κB活性和TGF-β1表达的抑制作用。结果表明，Fmod和Dcn的过表达与NF-κB和TGF-β1的下调有关，并且与Dcn相比，Fmod能更有效地促进这种作用。
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本文引用的文献
1
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J Cell Biochem. 2016 Jun;117(6):1279-87. doi: 10.1002/jcb.25496. Epub 2016 Feb 11.
2
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Sci Rep. 2014 Sep 18;4:6383. doi: 10.1038/srep06383.
3
Fibromodulin-deficiency alters temporospatial expression patterns of transforming growth factor-β ligands and receptors during adult mouse skin wound healing.纤维调节蛋白缺乏会改变成年小鼠皮肤伤口愈合过程中转化生长因子-β配体和受体的时空表达模式。
PLoS One. 2014 Mar 6;9(6):e90817. doi: 10.1371/journal.pone.0090817. eCollection 2014.
4
Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.人膀胱癌细胞缺乏 decorin 的表达为尿路上皮恶性肿瘤的治疗提供了新的工具。
PLoS One. 2013 Oct 11;8(10):e76190. doi: 10.1371/journal.pone.0076190. eCollection 2013.
5
The roles of TGFβ in the tumour microenvironment.TGFβ 在肿瘤微环境中的作用。
Nat Rev Cancer. 2013 Nov;13(11):788-99. doi: 10.1038/nrc3603. Epub 2013 Oct 17.
6
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Mol Cancer. 2013 Aug 2;12:86. doi: 10.1186/1476-4598-12-86.
7
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Histochem Cell Biol. 2013 Jan;139(1):161-71. doi: 10.1007/s00418-012-1026-0. Epub 2012 Sep 25.
8
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Oncogene. 2012 Sep 13;31(37):4150-63. doi: 10.1038/onc.2011.571. Epub 2011 Dec 12.
9
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PLoS One. 2011;6(10):e26432. doi: 10.1371/journal.pone.0026432. Epub 2011 Oct 19.
10
NF-κB, stem cells and breast cancer: the links get stronger.NF-κB、干细胞和乳腺癌：联系更加紧密。
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