Wang Jiazheng, Yan Meiying, Gao He, Lu Xin, Kan Biao
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
Appl Environ Microbiol. 2017 Jun 30;83(14). doi: 10.1128/AEM.00713-17. Print 2017 Jul 15.
is an important human pathogen and environmental microflora species that can both propagate in the human intestine and proliferate in zooplankton and aquatic organisms. Cholera is transmitted through food and water. In recent years, outbreaks caused by -contaminated soft-shelled turtles, contaminated mainly with toxigenic serogroup O139, have been frequently reported, posing a new foodborne disease public health problem. In this study, the colonization by toxigenic on the body surfaces and intestines of soft-shelled turtles was explored. Preferred colonization sites on the turtle body surfaces, mainly the carapace and calipash of the dorsal side, were observed for the O139 and O1 strains. Intestinal colonization was also found. The colonization factors of played different roles in the colonization of the soft-shelled turtle's body surface and intestine. Mannose-sensitive hemagglutinin (MSHA) of was necessary for body surface colonization, but no roles were found for toxin-coregulated pili (TCP) or -acetylglucosamine-binding protein A (GBPA). Both TCP and GBPA play important roles for colonization in the intestine, whereas the deletion of MSHA revealed only a minor colonization-promoting role for this factor. Our study demonstrated that can colonize the surfaces and the intestines of soft-shelled turtles and indicated that the soft-shelled turtles played a role in the transmission of cholera. In addition, this study showed that the soft-shelled turtle has potential value as an animal model in studies of the colonization and environmental adaption mechanisms of in aquatic organisms. Cholera is transmitted through water and food. Soft-shelled turtles contaminated with (commonly the serogroup O139 strains) have caused many foodborne infections and outbreaks in recent years, and they have become a foodborne disease problem. Except for epidemiological investigations, no experimental studies have demonstrated the colonization by on soft-shelled turtles. The present studies will benefit our understanding of the interaction between and the soft-shelled turtle. We demonstrated the colonization by on the soft-shelled turtle's body surface and in the intestine and revealed the different roles of major factors for colonization on the body surface and in the intestine. Our work provides experimental evidence for the role of soft-shelled turtles in cholera transmission. In addition, this study also shows the possibility for the soft-shelled turtle to serve as a new animal model for studying the interaction between and aquatic hosts.
是一种重要的人类病原体和环境微生物物种，既能在人类肠道中繁殖，也能在浮游动物和水生生物中增殖。霍乱通过食物和水传播。近年来，经常有报道称受污染的甲鱼引发疫情，主要被产毒血清群O139污染，这构成了一个新的食源性疾病公共卫生问题。在本研究中，探索了产毒霍乱弧菌在甲鱼体表和肠道的定殖情况。观察到O139和O1菌株在甲鱼体表的优先定殖部位，主要是背侧的甲壳和裙边。还发现了肠道定殖。霍乱弧菌的定殖因子在甲鱼体表和肠道定殖中发挥不同作用。霍乱弧菌的甘露糖敏感血凝素（MSHA）对于体表定殖是必需的，但毒素协同调节菌毛（TCP）或N - 乙酰葡糖胺结合蛋白A（GBPA）未发现有作用。TCP和GBPA在肠道定殖中都起重要作用，而MSHA的缺失仅显示该因子有轻微的促进定殖作用。我们的研究表明霍乱弧菌可以定殖在甲鱼的体表和肠道，并表明甲鱼在霍乱传播中起作用。此外，本研究表明甲鱼作为动物模型在霍乱弧菌在水生生物中的定殖和环境适应机制研究中具有潜在价值。霍乱通过水和食物传播。受霍乱弧菌（通常是血清群O139菌株）污染的甲鱼近年来已导致许多食源性感染和疫情爆发，它们已成为一个食源性疾病问题。除了流行病学调查外，没有实验研究证明霍乱弧菌在甲鱼上的定殖情况。目前的研究将有助于我们理解霍乱弧菌与甲鱼之间的相互作用。我们证明了霍乱弧菌在甲鱼体表和肠道的定殖，并揭示了主要霍乱弧菌因子在体表和肠道定殖中的不同作用。我们的工作为甲鱼在霍乱传播中的作用提供了实验证据。此外，本研究还表明甲鱼有可能作为研究霍乱弧菌与水生宿主之间相互作用的新动物模型。
