Zdorovenko Evelina L, Cimmino Alessio, Marchi Guido, Shashkov Alexander S, Fiori Mario, Knirel Yuriy A, Evidente Antonio
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
Dipartimento di Scienze Chimiche, Universitá di Napoli Federico II, I-80126 Naples, Italy.
Carbohydr Res. 2017 Aug 7;448:48-51. doi: 10.1016/j.carres.2017.05.019. Epub 2017 Jun 1.
An O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Pseudomonas mediterranea strain C5P1rad1, the causal agents of tomato pith necrosis and Chrysanthemum stem rot, and studied by one- and two-dimensional H and C NMR spectroscopy. The following structure of the trisaccharide repeating unit of the OPS was established, which, to our knowledge, is unique among the known bacterial polysaccharide structures: →4)-β-d-ManpNAc3NAcA-(1 → 4)-β-d-ManpNAc3NAcA-(1 → 3)-α-d-QuipNAc4NAc-(1→ where QuiNAc4NAc and ManNAc3NAcA indicate 2,4-diacetamido-2,4,6-trideoxyglucose and 2,3-diacetamido-2,3-dideoxymannuronic acid, respectively. Pre-treatment of leaves with LPS or OPS preparations at 250 and 50 μg mL did not inhibit development of a hypersensitivity reaction induced by P. mediterranea C5P1rad1 on tobacco, tomato and chrysanthemum plants. The same preparations at 250 μg mL partially prevented elicitation of the hypersensitivity reaction by Pseudomonas syringae KVPT7RC on chrysanthemum but not tobacco and tomato.
从地中海假单胞菌C5P1rad1菌株的脂多糖中分离出一种O-特异性多糖（OPS），该菌株是番茄髓坏死和菊花茎腐病的病原体，并通过一维和二维氢谱和碳谱核磁共振波谱对其进行了研究。确定了OPS三糖重复单元的以下结构，据我们所知，在已知的细菌多糖结构中，该结构是独特的：→4)-β-d-甘露糖胺3NAcA-(1→4)-β-d-甘露糖胺3NAcA-(1→3)-α-d-喹诺糖胺4NAc-(1→，其中喹诺糖胺4NAc和甘露糖胺3NAcA分别表示2,4-二乙酰氨基-2,4,6-三脱氧葡萄糖和2,3-二乙酰氨基-2,3-二脱氧甘露糖醛酸。用250和50μg/mL的LPS或OPS制剂预处理叶片，不会抑制地中海假单胞菌C5P1rad1对烟草、番茄和菊花植株诱导的过敏反应的发展。250μg/mL的相同制剂部分阻止了丁香假单胞菌KVPT7RC对菊花但不对烟草和番茄引发过敏反应。
