Nanotechnology Research Group (NANO(+)), Ladoke Akintola University of Technology, PMB 4000 Ogbomoso, Nigeria; Department of Science Laboratory Technology, Ladoke Akintola University of Technology, PMB 4000 Ogbomoso, Nigeria.
Nanotechnology Research Group (NANO(+)), Ladoke Akintola University of Technology, PMB 4000 Ogbomoso, Nigeria; Laboratory of Industrial Microbiology and Nanobiotechnology, Ladoke Akintola University of Technology, PMB 4000 Ogbomoso, Nigeria; Department of Pure and Applied Biology, Ladoke Akintola University of Technology, PMB 4000 Ogbomoso, Nigeria.
J Photochem Photobiol B. 2017 Aug;173:250-257. doi: 10.1016/j.jphotobiol.2017.06.003. Epub 2017 Jun 3.
In the current work, cell-free extracts of four strains of non-pathogenic Enterococcus species of food origin, were studied for the green synthesis of gold nanoparticles (AuNPs), and characterized by UV-Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The AuNPs were evaluated for their Anopheles gambiae larvicidal, dye degradation, antioxidant and thrombolytic activities. The blue-black colloidal AuNPs which absorbed maximally at 549-552nm were nearly spherical in shape, and crystalline in nature with size of 8-50nm. The EDX spectra showed formation of AuNPs to the tune of 89-94%. The prominent FTIR peaks obtained at 3251-3410, 2088 and 1641-1643cm alluded to the fact that proteins were involved in the biofabrication and capping of AuNPs. AuNPs degraded methylene blue and malachite green by 24.3-57.6%, and 88.85-97.36% respectively in 24h, whereas at 12h, larvicidal activities with LC of 21.28-42.33μg/ml were obtained. DPPH scavenging activities of 33.24-51.47% were obtained for the biosynthesized AuNPs. The AuNPs prevented coagulation of blood and also achieved 9.4-94.6% lysis of blood clot showing potential nanomedical applications. This study has presented an eco-friendly and economical synthesis of AuNPs by non-pathogenic strains of Enterococcus species for various nanobiotechnological applications.
在当前的工作中，研究了来自食品源的四种非致病性肠球菌菌株的无细胞提取物，以用于金纳米粒子（AuNPs）的绿色合成，并通过紫外-可见吸收光谱、傅里叶变换红外光谱（FTIR）和透射电子显微镜（TEM）进行了表征。评估了 AuNPs 对疟蚊幼虫的杀虫、染料降解、抗氧化和溶栓活性。最大吸收在 549-552nm 的蓝黑色胶体 AuNPs 呈近球形，且为纳米级 8-50nm 的晶体。EDX 光谱显示 AuNPs 的形成率为 89-94%。在 3251-3410、2088 和 1641-1643cm 处获得的显著 FTIR 峰表明，蛋白质参与了 AuNPs 的生物制造和封端。AuNPs 在 24h 内分别将亚甲基蓝和孔雀石绿降解了 24.3-57.6%和 88.85-97.36%，而在 12h 时，获得了 LC 为 21.28-42.33μg/ml 的杀虫活性。生物合成的 AuNPs 的 DPPH 清除活性为 33.24-51.47%。AuNPs 可防止血液凝固，并且还可实现 9.4-94.6%的血凝块溶解，显示出潜在的纳米医学应用。本研究通过非致病性肠球菌菌株提出了一种环保且经济的 AuNPs 合成方法，可用于各种纳米生物技术应用。
