Laboratory of Industrial Microbiology and Nanobiotechnology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Biotechnol Prog. 2019 Sep;35(5):e2829. doi: 10.1002/btpr.2829. Epub 2019 May 20.
The search for biocompatible nanoparticles with vast applicability has impacted on exploration of various biomaterials for the synthesis of mono and bimetallic nanoparticles. Xylanase is widely regarded as an industrially important enzyme but its potentials in nanotechnological applications are yet to be fully explored. The current study investigates the exploit of xylanases of Aspergillus niger L3 (NE) and Trichoderma longibrachiatum L2 (TE) produced through valorization of corn-cob, to synthesize silver-gold alloy nanoparticles (Ag-AuNPs). Characterization of the Ag-AuNPs involved UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy and transmission electron microscopy, while their prospective use as antimicrobial, antioxidant, catalytic, anticoagulant, and thrombolytic agents were studied. The biosynthesized Ag-AuNPs were ruby red and light purple with surface plasmon resonance at 520 and 534 nm for NEAg-AuNPs and TEAg-AuNPs, respectively; while FTIR showed that protein molecules capped and stabilized the nanoparticles. The Ag-AuNPs were anisotropic with spherical, oval, and irregular shapes having sizes ranging from 6.98 to 52.51 nm. The nanoparticles appreciably inhibited the growth of tested clinical bacteria (23.40-90.70%) and fungi (70.10-89.05%), and also scavenged 2,2-diphenyl-1-picrylhydrazyl (48.51-53.79%) and hydrogen peroxide (80.5-95.50%). Furthermore, the Ag-AuNPs degraded malachite green (91.39%) and methylene blue (47.10%). Moreover, the Ag-AuNPs displayed outstanding anticoagulant and thrombolytic activities using human blood. This study further emphasizes the significance of xylanases in nanobiotechnology as it has established the potential of xylanases to synthesize Ag-AuNPs, which is being reported for the first time.
寻找具有广泛适用性的生物相容性纳米粒子已经影响到各种生物材料的探索，以合成单金属和双金属纳米粒子。木聚糖酶被广泛认为是一种具有工业重要性的酶，但它在纳米技术应用中的潜力尚未得到充分探索。本研究利用通过玉米芯增值生产的黑曲霉 L3（NE）和长枝木霉 L2（TE）的木聚糖酶，来合成银-金合金纳米粒子（Ag-AuNPs）。Ag-AuNPs 的表征涉及紫外-可见光谱、傅里叶变换红外光谱（FTIR）和场发射扫描电子显微镜和透射电子显微镜，同时研究了它们作为抗菌、抗氧化、催化、抗凝和溶栓剂的潜在用途。生物合成的 Ag-AuNPs 呈红宝石红色和浅紫色，表面等离子体共振分别为 520nm 和 534nm；而 FTIR 表明，蛋白质分子覆盖并稳定了纳米粒子。Ag-AuNPs 具有各向异性，形状为球形、椭圆形和不规则形，尺寸范围为 6.98nm 至 52.51nm。纳米粒子显著抑制了测试的临床细菌（23.40%-90.70%）和真菌（70.10%-89.05%）的生长，还清除了 2,2-二苯基-1-苦基肼（48.51%-53.79%）和过氧化氢（80.5%-95.50%）。此外，Ag-AuNPs 还降解了孔雀石绿（91.39%）和亚甲基蓝（47.10%）。此外，Ag-AuNPs 在人血中表现出出色的抗凝和溶栓活性。本研究进一步强调了木聚糖酶在纳米生物技术中的重要性，因为它已经确立了木聚糖酶合成 Ag-AuNPs 的潜力，这是首次报道。
