Xin Miaomiao, Siddique Mohammad Abdul Momin, Dzyuba Borys, Cuevas-Uribe Rafael, Shaliutina-Kolešová Anna, Linhart Otomar
Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research, Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic.
Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research, Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic; Department of Oceanography, Noakhali Science and Technology University, Sonapur, Noakhali, Bangladesh.
Theriogenology. 2017 Aug;98:16-22. doi: 10.1016/j.theriogenology.2017.04.043. Epub 2017 Apr 27.
To survive low temperature is required for a long-term storage (cryopreservation), cells should be vitrified to a state in which intracellular water is solidified without ice crystal formation. Two different approaches are described for fish sperm cryopreservation: 1) sperm conventional cryopreservation, in which extracellular water is partially crystallized and 2) sperm vitrification, in which both intra- and extra-cellular liquids are vitrified. Sperm vitrification has been applied to some fish species with limited success. Traditional vitrification requires rapid cooling/warming rates, small sample carriers, and using high permeable cryoprotectant concentrations. The latter cause cytotoxic effects which must be well managed and will require continuous effort to match an appropriate cryoprotectant with suitable apparatus and warming methods. Novel cryoprotectant-free sperm vitrification approach has been applied to several fishes. This review summarizes development of basic procedures and discusses advantages and disadvantages of vitrification when applied it to fish sperm.
为了长期保存（冷冻保存）而在低温下存活，细胞应被玻璃化至细胞内水固化而不形成冰晶的状态。描述了两种用于鱼类精子冷冻保存的不同方法：1）精子常规冷冻保存，其中细胞外水部分结晶；2）精子玻璃化，其中细胞内和细胞外液体均被玻璃化。精子玻璃化已应用于一些鱼类，但成功率有限。传统的玻璃化需要快速的冷却/升温速率、小的样品载体以及使用高渗透性的冷冻保护剂浓度。后者会产生细胞毒性作用，必须妥善管理，并且需要不断努力将合适的冷冻保护剂与合适的仪器和升温方法相匹配。新型无冷冻保护剂的精子玻璃化方法已应用于几种鱼类。本综述总结了基本程序的发展，并讨论了将玻璃化应用于鱼类精子时的优缺点。
