Shikichi Mitsumori, Iwata Katsuroku, Ito Katsumi, Miyakoshi Daisuke, Murase Harutaka, Sato Fumio, Korosue Kenji, Nagata Shunichi, Nambo Yasuo
Elm Equine Clinic, 59-61 Midorimachi, Urakawa, Hokkaido 057-0025, Japan.
NOSAI-Hidaka, 1-17-13 Sakaimachinishi, Urakawa, Hokkaido 057-0034, Japan.
Theriogenology. 2017 Aug;98:75-81. doi: 10.1016/j.theriogenology.2017.04.024. Epub 2017 Apr 21.
Measurement of progestines and estrogens in maternal plasma has been advocated as an indicator of foeto-placental function. The goals of the present study were as follows: to determine progestin and estrogen concentrations in mares with normal and abnormal pregnancies during late gestation, evaluate the diagnostic value of hormone concentrations, and investigate the relationship between hormone concentrations after treatment and the survival of foals. Blood samples were collected monthly from 459 pregnant mares between Day 201 of gestation and foaling. Progestin and estrogen concentrations were measured using a time-resolved fluoroimmunoassay with anti-progesterone and anti-17β-estradiol antibodies. The cutoff values of hormone concentrations that best discriminated between healthy foals and foal loss groups were determined using receiver-operating characteristic (ROC) curve analysis. Mares with compromised pregnancies are treated with progestins, tocolytics and antibiotics according to clinical signs, ultrasonographic examination and hormonal test. We investigated the relationship between the restoration of hormone concentrations after treatment and the survival rate of the foals of 62 compromised mares. Maternal serum progestin and estrogen concentrations on Days 241-320 of gestation differed significantly between healthy foals and foal loss of each group. Predictions of pregnancy outcomes were based on ROC curve analysis that established cutoff values of serum progestin and estrogen concentrations for each 20-day interval during gestation. All mares with improved concentrations of progestins or estrogens after treatment delivered healthy foals. When hormone concentrations got worse after treatment, the rate of loss was higher when treatment was administered during Days 201-290 of gestation than after Day 290. Mares with high progestin and low estrogen concentrations were likely to deliver aborted/dead foals during mid-to-late gestation. These results suggest that measurement of progestin and estrogen concentrations may be useful for diagnosing abnormal pregnancy and implementing early treatment strategies.
测定母马血浆中的孕激素和雌激素被认为是评估胎儿 - 胎盘功能的一项指标。本研究的目的如下：测定妊娠晚期正常和异常妊娠母马的孕激素和雌激素浓度，评估激素浓度的诊断价值，并研究治疗后激素浓度与驹的存活率之间的关系。在妊娠第201天至分娩期间，每月从459匹怀孕母马采集血样。使用抗孕酮和抗17β - 雌二醇抗体的时间分辨荧光免疫分析法测定孕激素和雌激素浓度。使用受试者操作特征（ROC）曲线分析确定能最佳区分健康驹和驹丢失组的激素浓度临界值。根据临床症状、超声检查和激素检测，对妊娠受损的母马使用孕激素、宫缩抑制剂和抗生素进行治疗。我们研究了62匹妊娠受损母马治疗后激素浓度恢复与驹存活率之间的关系。每组中，健康驹和驹丢失组在妊娠第241 - 320天的母马血清孕激素和雌激素浓度存在显著差异。妊娠结局的预测基于ROC曲线分析，该分析确定了妊娠期间每20天间隔的血清孕激素和雌激素浓度临界值。所有治疗后孕激素或雌激素浓度改善的母马都产下了健康的驹。当治疗后激素浓度变差时，在妊娠第201 - 290天进行治疗时的丢失率高于第290天之后。孕激素浓度高且雌激素浓度低的母马在妊娠中后期可能产下流产/死胎。这些结果表明，测定孕激素和雌激素浓度可能有助于诊断异常妊娠并实施早期治疗策略。
