Wang Shuqi E, Brooks Anna E S, Cann Bronwyn, Simoes-Barbosa Augusto
School of Biological Sciences, University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand.
School of Biological Sciences, University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand; Maurice Wilkins Centre, University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand.
Mol Biochem Parasitol. 2017 Sep;216:1-4. doi: 10.1016/j.molbiopara.2017.06.003. Epub 2017 Jun 9.
Trichomonas vaginalis is a flagellated protozoan causing a notorious urogenital infection in humans. Due to its anaerobic metabolism, an alternative fluorescent protein that can be readily expressed in oxygen-deprived conditions is ideal. This study assessed the performance of iLOV, which does not require oxygen to function, as compared to the conventional enhanced green fluorescent protein (eGFP) in T. vaginalis. The results indicated that iLOV outperforms eGFP in both transient and stable expression, being detectable earlier and producing higher fluorescent intensity than eGFP in T. vaginalis. This finding facilitates forthcoming genetic studies that will advance the knowledge on this human parasitic infection.
阴道毛滴虫是一种有鞭毛的原生动物，可引起人类臭名昭著的泌尿生殖系统感染。由于其厌氧代谢，一种能在缺氧条件下轻松表达的替代性荧光蛋白是理想之选。本研究评估了与传统增强型绿色荧光蛋白（eGFP）相比，在阴道毛滴虫中无需氧气即可发挥作用的iLOV的性能。结果表明，在瞬时表达和稳定表达方面，iLOV均优于eGFP，在阴道毛滴虫中比eGFP更早可检测到且产生更高的荧光强度。这一发现有助于即将开展的遗传学研究，从而增进对这种人类寄生虫感染的了解。
