Jung Yong-Taek, Park Sooyeon, Lee Jung-Sook, Yoon Jung-Hoon
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1855-1860. doi: 10.1099/ijsem.0.001877. Epub 2017 Jun 12.
A Gram-stain-negative, short-rod-shaped, non-motile bacterial strain, designated HWR-46T, was isolated from seawater of the Yellow Sea, South Korea, and was subjected to a polyphasic taxonomic study. Strain HWR-46T grew optimally at pH 7.0-8.0, at 20-25 °C and in the presence of 2-3 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HWR-46T fell within the clade comprising Pseudorhodobacter species, clustering with the type strain of Pseudorhodobacter aquimaris, with which it exhibited 98.4 % sequence similarity. 16S rRNA gene sequence similarity between strain HWR-46T and the type strains of other Pseudorhodobacter species was 95.1-96.3 %. The DNA G+C content of strain HWR-46T was 63.1 mol% and its mean DNA-DNA relatedness value with P. aquimaris HDW-19T was 24.6±1.5 %. Strain HWR-46T contained only Q-10 as the ubiquinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and an unidentified aminophospholipid. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain HWR-46T is distinguishable from other Pseudorhodobacter species. On the basis of the data presented, strain HWR-46T is considered to represent a novel species of the genus Pseudorhodobacter, for which the name Pseudorhodobacter ponti sp. nov. is proposed. The type strain is HWR-46T (=KCTC 52470T=NBRC 112426T).
从韩国黄海海水中分离出一株革兰氏染色阴性、短杆状、无运动性的细菌菌株，命名为HWR-46T，并对其进行了多相分类研究。菌株HWR-46T在pH 7.0 - 8.0、20 - 25 °C以及2 - 3 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株HWR-46T属于包含假红杆菌属物种的进化枝，与海假红杆菌模式菌株聚类在一起，序列相似性为98.4 %。菌株HWR-46T与其他假红杆菌属物种模式菌株之间的16S rRNA基因序列相似性为95.1 - 96.3 %。菌株HWR-46T的DNA G+C含量为63.1 mol%，其与海假红杆菌HDW-19T的平均DNA-DNA相关性值为24.6±1.5 %。菌株HWR-46T仅含有泛醌Q-10，主要脂肪酸为总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）。主要极性脂为磷脂酰胆碱、磷脂酰甘油、一种未鉴定的氨基脂和一种未鉴定的氨基磷脂。不同的表型特征以及系统发育独特性表明，菌株HWR-46T可与其他假红杆菌属物种区分开来。根据所提供的数据，菌株HWR-46T被认为代表假红杆菌属的一个新物种，为此提出新名称海假红杆菌（Pseudorhodobacter ponti sp. nov.）。模式菌株为HWR-4 6T（=KCTC 52470T=NBRC 112426T）。
