Song Jaeho, Jeon Hyoung Tae, Lim Yeonjung, Joung Yochan, Cho Jang-Cheon
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3260-3265. doi: 10.1099/ijsem.0.002977. Epub 2018 Aug 29.
A Gram-stain-negative, non-flagellated, motile-by-gliding, orange-coloured bacterial strain, designated strain IMCC20180, was isolated from seawater collected off the coast of the East Sea in the Republic of Korea. The 16S rRNA gene sequence analysis showed that strain IMCC20180 was most closely related to Winogradskyellaporiferorum UST030701-295 (95.7 % sequence similarity) and formed a robust phylogenetic clade with other Winogradskyella species, indicating that the strain was affiliated with the genus Winogradskyella. Growth of strain IMCC20180 was observed at 20-30 °C (optimum, 25 °C), pH 7.0-9.0 (pH 8.0) and with 1.0-3.5 % NaCl (3.0 %, w/v). The predominant isoprenoid quinone was MK-6. Major fatty acid constituents were iso-C15 : 1 G, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), iso-C15 : 0, iso-C17 : 0 3-OH and iso-C16 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, four unknown aminolipids and five unknown lipids. The estimated genome size and the DNA G+C content of strain IMCC20180 were 3.1 Mb and 37.7 %, respectively. Based on 16S rRNA gene phylogeny, physiological and chemotaxonomic characterization, strain IMCC20180 represented a novel species in the genus Winogradskyella, for which the name Winogradskyellaaurantiaca sp. nov. is proposed. The type strain is IMCC20180 (=KACC 18883=KCTC 52240=JCM 31383).
从韩国东海沿岸采集的海水中分离出一株革兰氏染色阴性、无鞭毛、靠滑动运动、橙色的细菌菌株，命名为IMCC20180菌株。16S rRNA基因序列分析表明，IMCC20180菌株与多孔温诺格拉德氏菌UST030701-295关系最为密切（序列相似性为95.7%），并与其他温诺格拉德氏菌属物种形成了一个稳健的系统发育分支，表明该菌株属于温诺格拉德氏菌属。在20-30°C（最适温度为25°C）、pH 7.0-9.0（pH 8.0）和1.0-3.5%（w/v）NaCl条件下观察到IMCC20180菌株生长。主要类异戊二烯醌为MK-6。主要脂肪酸成分是异-C15:1 G、总和特征3（C16:1ω6c和/或C16:1ω7c）、异-C15:0、异-C17:0 3-OH和异-C16:0 3-OH。主要极性脂类为磷脂酰乙醇胺、四种未知氨基脂类和五种未知脂类。IMCC20180菌株的估计基因组大小和DNA G+C含量分别为3.1 Mb和37.7%。基于16S rRNA基因系统发育学、生理学和化学分类学特征，IMCC20180菌株代表了温诺格拉德氏菌属中的一个新物种，为此提出了新名称橙色温诺格拉德氏菌（Winogradskyella aurantiaca sp. nov.）。模式菌株为IMCC20180（=KACC 18883=KCTC 52240=JCM 31383）。
