School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.
Lab Chip. 2017 Jun 27;17(13):2193-2197. doi: 10.1039/c7lc00164a.
We report a novel fusion of droplet microfluidics and light-sheet microscopy, to achieve high-throughput sample compartmentalization, manipulation and three-dimensional imaging on a chip. This optofluidic device characterized by orthogonal plane illumination and rapid liquid handling is compact and cost-effective, and capable of preparing sample droplets with tunable size, frequency and ingredient. Each droplet flowing through the device's imaging region is self-scanned by a laser-sheet, three-dimensionally reconstructed and quantitatively analysed. This simple-and-robust platform combines fast 3-D imaging with efficient sample preparation and eliminates the need of a complicated mechanical scan at the same time. Achieving 500 measurements per second and screening over 30 samples per minute, it shows great potential for various lab-on-a-chip biological studies, such as embryo sorting and cell growth assays.
我们报告了一种新的液滴微流控和光片显微镜融合技术，可在芯片上实现高通量样品分隔、操作和三维成像。这种具有正交平面照明和快速液体处理功能的光电混合装置结构紧凑、经济高效，能够制备具有可调大小、频率和成分的样品液滴。每个流过器件成像区域的液滴都由激光片自扫描、三维重建和定量分析。这种简单而稳健的平台将快速 3D 成像与高效的样品制备相结合，同时消除了对复杂机械扫描的需求。该平台实现了每秒 500 次测量和每分钟 30 多个样本的筛选，在各种微流控芯片生物研究中具有巨大的潜力，如胚胎分选和细胞生长分析。
