便携式光片流式光学显微镜用于三维荧光成像流式细胞术。

Portable light-sheet optofluidic microscopy for 3D fluorescence imaging flow cytometry.

机构信息

Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.

Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.

出版信息

Lab Chip. 2023 Feb 14;23(4):624-630. doi: 10.1039/d2lc01024k.

DOI:10.1039/d2lc01024k

PMID:36633262

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9931680/

Abstract

Imaging flow cytometry (IFC) combines conventional flow cytometry with optical microscopy, allowing for high-throughput, multi-parameter screening of single-cell specimens with morphological and spatial information. However, current 3D IFC systems are limited by instrumental complexity and incompatibility with available microfluidic devices or operations. Here, we report portable light-sheet optofluidic microscopy (PLSOM) for 3D fluorescence cytometric imaging. PLSOM exploits a compact, open-top light-sheet configuration compatible with commonly adopted microfluidic chips. The system offers a subcellular resolution (2-4 μm) in all three dimensions, high throughput (∼1000 cells per s), and portability (30 cm () × 10 cm () × 26 cm ()). We demonstrated PLSOM for 3D IFC using various phantom and cell systems. The low-cost and custom-built architecture of PLSOM permits easy adaptability and dissemination for broad 3D flow cytometric investigations.

摘要

成像流式细胞术（IFC）将传统流式细胞术与光学显微镜相结合，可对具有形态和空间信息的单细胞标本进行高通量、多参数筛选。然而，当前的 3D IFC 系统受到仪器复杂性和与现有微流控设备或操作不兼容的限制。在这里，我们报告了用于 3D 荧光细胞计量成像的便携式光片光流体显微镜（PLSOM）。PLSOM 利用与常用微流控芯片兼容的紧凑型、开放式光片配置。该系统在所有三个维度上均提供亚细胞分辨率（2-4 μm）、高通量（∼1000 个细胞/秒）和便携性（30 cm()×10 cm()×26 cm()）。我们使用各种幻影和细胞系统展示了 PLSOM 用于 3D IFC。PLSOM 的低成本和定制构建架构允许轻松适应和传播，以进行广泛的 3D 流式细胞术研究。