Azizieh Fawaz, Raghupathy Raj, Shehab Diaa, Al-Jarallah Khaled, Gupta Renu
1Department of Mathematics and Natural Sciences, Gulf University for Science and Technology, Kuwait 2Department of Microbiology 3Department of Medicine 4Department of Radiology, Faculty of Medicine, Kuwait University, Kuwait.
Menopause. 2017 Sep;24(9):1057-1064. doi: 10.1097/GME.0000000000000885.
As the immune system is suggested to contribute to the pathophysiology of osteoporosis in menopause, we compared the levels of proresorptive and antiresorptive cytokines produced by peripheral blood mononuclear cells (PBMCs) from postmenopausal women with normal and low bone mineral density (BMD).
Seventy-one postmenopausal women were studied; 25 had normal BMD and 46 had low BMD. Participants were categorized as normal (n = 25), osteopenic (n = 31), and osteoporotic (n = 15) based on T-scores. Levels of 10 cytokines produced by mitogen-stimulated PBMCs were measured by Multiplex ELISA.
PBMCs from women with low BMD produced higher levels of the proresorptive cytokines tumor necrosis factor-alpha, interleukin (IL)-6, IL-12, and IL-17 (P = 0.014, 0.012, 0.011, and 0.049), and lower levels of the antiresorptive cytokines IL-4, IL-10, and IL-23 (P = 0.003, 0.018, and 0.025) compared with women with normal BMD. Proresorptive cytokines were similar in osteopenic and osteoporotic women, but both had higher levels than women with normal BMD. Osteoporotic women produced lower levels of the antiresorptive cytokines IL-4, IL-10, IL-13, and IL-23 compared with the normal BMD group (P = 0.001, 0.05, 0.05, and 0.026), and lower levels of IL-4 as compared with osteopenic women (P = 0.05). Osteopenic women produced lower levels of IL-4 and IL-10 compared with the normal BMD group (P = 0.025 and 0.038). Ratios of proresorptive to antiresorptive cytokines suggest a stronger proresorptive cytokine bias in women with low BMD. Most of the ratios are lowest in the normal BMD group, modest in osteopenic women, and highest in the osteoporotic group.
Women with low BMD have a proresorptive cytokine bias.
鉴于免疫系统被认为与绝经后骨质疏松症的病理生理过程有关，我们比较了骨矿物质密度（BMD）正常和偏低的绝经后女性外周血单核细胞（PBMCs）产生的促吸收和抗吸收细胞因子水平。
对71名绝经后女性进行了研究；其中25名BMD正常，46名BMD偏低。根据T值将参与者分为正常组（n = 25）、骨量减少组（n = 31）和骨质疏松组（n = 15）。通过多重酶联免疫吸附测定法（Multiplex ELISA）测量有丝分裂原刺激的PBMCs产生的10种细胞因子水平。
与BMD正常的女性相比，BMD偏低的女性的PBMCs产生的促吸收细胞因子肿瘤坏死因子-α、白细胞介素（IL）-6、IL-12和IL-17水平更高（P = 0.014、0.012、0.011和0.049），而抗吸收细胞因子IL-4、IL-10和IL-23水平更低（P = 0.003、0.018和0.025）。骨量减少和骨质疏松女性的促吸收细胞因子水平相似，但均高于BMD正常的女性。与BMD正常组相比，骨质疏松女性产生的抗吸收细胞因子IL-4、IL-10、IL-13和IL-23水平更低（P = 0.001、0.05、0.05和0.026），与骨量减少女性相比，IL-4水平更低（P = 0.05）。与BMD正常组相比，骨量减少女性产生IL-4和IL-10的水平更低（P = 0.025和0.038）。促吸收细胞因子与抗吸收细胞因子的比率表明，BMD偏低的女性中促吸收细胞因子的偏向更强。大多数比率在BMD正常组中最低，在骨量减少女性中适中，在骨质疏松组中最高。
BMD偏低的女性存在促吸收细胞因子偏向。
