Barranco Isabel, Perez-Patiño Cristina, Tvarijonaviciute Asta, Parrilla Inmaculada, Vicente-Carrillo Alejandro, Alvarez-Rodriguez Manuel, Ceron Jose J, Martinez Emilio A, Rodriguez-Martinez Heriberto, Roca Jordi
Department of Medicine and AnimalSurgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain.
Department of Clinical and Experimental Medicine (IKE), University of Linköping, Linköping, Sweden.
Reproduction. 2017 Sep;154(3):237-243. doi: 10.1530/REP-17-0300. Epub 2017 Jun 13.
The paraoxonase type 1 (PON1) is an enzyme with antioxidant properties recently identified in the seminal plasma (SP) of several species, including the porcine. The aims of the present study were to (1) describe the immunohistochemical localisation of PON1 in the genital organs of fertile boars and (2) evaluate the relationship among PON1 activity and high-density lipoprotein cholesterol (HDL-C) concentration in fluids of the boar genital organs. Immunohistochemical analysis demonstrated that PON1 was present in testis (specifically in Leydig cells, blood vessels, spermatogonia and elongated spermatids), epididymis (specifically in the cytoplasm of the principal epithelial cells, luminal secretion and in the surrounding smooth muscle) and the lining epithelia of the accessory sexual glands (cytoplasmic location in the prostate and membranous in the seminal vesicle and bulbourethral glands). The Western blotting analysis confirmed the presence of PON1 in all boar genital organs, showing in all of them a band of 51 kDa and an extra band of 45 kDa only in seminal vesicles. PON1 showed higher activity levels in epididymal fluid than those in SP of the entire ejaculate or of specific ejaculate portions. A highly positive relationship between PON1 activity and HDL-C concentration was found in all genital fluids. In sum, all boar genital organs contributing to sperm-accompanying fluid/s were able to express PON1, whose activity in these genital fluids is highly dependent on the variable HDL-C concentration present.
对氧磷酶1（PON1）是一种具有抗氧化特性的酶，最近在包括猪在内的几种物种的精浆（SP）中被发现。本研究的目的是：（1）描述PON1在可育公猪生殖器官中的免疫组织化学定位；（2）评估公猪生殖器官液体中PON1活性与高密度脂蛋白胆固醇（HDL-C）浓度之间的关系。免疫组织化学分析表明，PON1存在于睾丸（特别是在睾丸间质细胞、血管、精原细胞和延长型精子细胞中）、附睾（特别是在主上皮细胞的细胞质、管腔分泌物以及周围平滑肌中）和附属性腺的衬里上皮（在前列腺中位于细胞质，在精囊和尿道球腺中位于膜部）。蛋白质免疫印迹分析证实了所有公猪生殖器官中都存在PON1，在所有器官中均显示出一条51 kDa的条带，而仅在精囊中出现一条45 kDa的额外条带。PON1在附睾液中的活性水平高于整个射精或特定射精部分的精浆中的活性水平。在所有生殖液体中均发现PON1活性与HDL-C浓度之间存在高度正相关。总之，所有对公猪伴随精子的液体有贡献的生殖器官都能够表达PON1，其在这些生殖液体中的活性高度依赖于其中存在的可变HDL-C浓度。
