Centre de Biophysique Moléculaire , CNRS UPR 4301, 45071 Orléans Cedex 2, France.
Department of Chemistry, Willard H. Dow Laboratories, University of Michigan , Ann Arbor, Michigan 48109, United States.
J Am Chem Soc. 2017 Jun 28;139(25):8388-8391. doi: 10.1021/jacs.7b01587. Epub 2017 Jun 14.
Sensitive detection of cell necrosis is crucial for the determination of cell viability. Because of its high resolution at the cellular level and sensitivity, optical imaging is highly attractive for identifying cell necrosis. However, challenges associated with this technique remain present such as the rapid photobleaching of several types of organic fluorophores and/or the interference generated by biological autofluorescence. Herein, we synthesized novel biologically compatible Zn/Ln metallacrowns (MCs) that possess attractive near-infrared (NIR) emission and are highly photostable. In addition, these MCs have the ability to label differentially necrotic HeLa cells from living cells. This work is also the first demonstration of (i) the use of the NIR emission arising from a single lanthanide(III) cation for optical biological imaging of cells under single photon excitation, (ii) the first example of a lanthanide(III)-based NIR-emitting probe that can be targeted to a specific type of cell.
细胞坏死的灵敏检测对于确定细胞活力至关重要。由于在细胞水平上具有高分辨率和灵敏度，光学成像是识别细胞坏死的理想选择。然而，该技术仍然存在一些挑战，例如几种有机荧光团的快速光漂白和/或生物自发荧光产生的干扰。在此，我们合成了新型生物相容性 Zn/Ln 金属冠（MC），具有吸引人的近红外（NIR）发射和高度光稳定性。此外，这些 MC 能够区分标记坏死的 HeLa 细胞和活细胞。这项工作也是首次证明了 (i) 在单光子激发下，利用单个镧系(III)阳离子产生的近红外发射进行细胞光学生物成像，(ii) 首例基于镧系(III)的近红外发射探针可靶向特定类型的细胞。
