Zhao Yang, Eldridge Will J, Maher Jason R, Kim Sanghoon, Crose Michael, Ibrahim Mohamed, Levinson Howard, Wax Adam
Opt Lett. 2017 Jun 15;42(12):2302-2305. doi: 10.1364/OL.42.002302.
We have developed dual-axis optical coherence tomography (DA-OCT) which enables deep tissue imaging by using a novel off-axis illumination/detection configuration. DA-OCT offers a 100-fold speed increase compared with its predecessor, multispectral multiple-scattering low coherence interferometry (ms2/LCI), by using a new beam scanning mechanism based on a microelectro-mechanical system (MEMS) mirror. The data acquisition scheme was altered to take advantage of this scanning speed, producing tomographic images at a rate of 4 frames (B-scans) per second. DA-OCT differs from ms2/LCI in that the dual axes intersect at a shallower depth (∼1 mm). This difference, coupled with the faster scanning speed, shifts the detection priority from multiply scattered to ballistic light. The utility of this approach was demonstrated by imaging both ex vivo porcine ear skin and in vivo rat skin from a McFarlane flap model. The enhanced penetration depth provided by the DA-OCT system will be beneficial to various clinical applications in dermatology and surgery.
我们开发了双轴光学相干断层扫描技术（DA-OCT），它通过使用一种新颖的离轴照明/检测配置实现深度组织成像。与它的前身多光谱多重散射低相干干涉测量技术（ms2/LCI）相比，DA-OCT通过使用基于微机电系统（MEMS）镜的新型光束扫描机制，速度提高了100倍。数据采集方案进行了改变以利用这种扫描速度，每秒以4帧（B扫描）的速率生成断层图像。DA-OCT与ms2/LCI的不同之处在于，双轴在较浅深度（约1毫米）相交。这种差异，再加上更快的扫描速度，将检测优先级从多重散射光转移到弹道光。通过对离体猪耳皮肤和来自麦克法兰皮瓣模型的活体大鼠皮肤进行成像，证明了这种方法的实用性。DA-OCT系统提供的增强穿透深度将有利于皮肤病学和外科的各种临床应用。
