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Construction in vitro of "phage-plasmid" chimerae: a new tool to analyse the mechanism of plasmid maintenance.

作者信息

Couturier M, Janssens J, Bex F, Desmyter A, Bonnevalle I

出版信息

Mol Gen Genet. 1979 Jan 16;169(1):113-6. doi: 10.1007/BF00267552.

DOI:10.1007/BF00267552
PMID:286146
Abstract

In this paper, we report the construction in vitro of chimerae between lambdoid replacement vectors (Murray et al., 1977) and the miniF Apr plasmid: pSC138 (Timmis et al., 1975). lambdaF recombinants were shown to be chimerae between the lambda and the F replicons. By genetical tests, we have demonstrated that both lambda and F replication mechanisms are functional: the lambdaF recombinant behaves as a non defective plaque forming phage on lambda sensitive bacteria and establishes itself as a stable plasmid on recA F- homoimmune bacteria. In the extra-chromosomal state, the lambdaF recombinant apparently retains the controlled autonomous replication and the FI incompatibility characteristics of the F plasmid. The potential experimental uses of these phages are discussed.

摘要

相似文献

1
Construction in vitro of "phage-plasmid" chimerae: a new tool to analyse the mechanism of plasmid maintenance.
Mol Gen Genet. 1979 Jan 16;169(1):113-6. doi: 10.1007/BF00267552.
2
Construction and some properties of packageable plasmid F.可包装质粒F的构建及其某些特性
Mol Gen Genet. 1979 Jan 16;169(1):107-12. doi: 10.1007/BF00267551.
3
Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda.构建含有噬菌体λ复制起点的嵌合噬菌体和质粒。
Science. 1977 Dec 9;198(4321):1041-6. doi: 10.1126/science.929185.
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DNA fusion product of phage P2 with plasmid pBR322: a new phasmid.噬菌体P2与质粒pBR322的DNA融合产物：一种新的噬菌粒。
Mol Gen Genet. 1983;189(2):343-7. doi: 10.1007/BF00337829.
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Construction of a hybrid bacteriophage-plasmid recombinant DNA vector.一种噬菌体 - 质粒杂交重组DNA载体的构建。
J Bacteriol. 1978 Dec;136(3):1192-6. doi: 10.1128/jb.136.3.1192-1196.1978.
6
Lambda phage promoter used to enhance expression of a plasmid-cloned gene.用于增强质粒克隆基因表达的λ噬菌体启动子。
Mol Gen Genet. 1978 Jul 11;163(2):197-203. doi: 10.1007/BF00267410.
7
Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein.新型质粒-噬菌体杂种的构建：利用该杂种在无ColE1特异性蛋白的情况下体内证明ColE1 DNA复制。
Proc Natl Acad Sci U S A. 1978 May;75(5):2200-4. doi: 10.1073/pnas.75.5.2200.
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Aberrant immunity behaviour of hybrid lambda imm21 phages containing the DNA of ColE1-type plasmids.含有ColE1型质粒DNA的杂交λimm21噬菌体的异常免疫行为。
Mol Gen Genet. 1979;172(3):329-37. doi: 10.1007/BF00271733.
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Replication of the plasmid pBR322 under the control of a cloned replication origin from the single-stranded DNA phage M13.在来自单链DNA噬菌体M13的克隆复制起点控制下的质粒pBR322的复制。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4638-42. doi: 10.1073/pnas.77.8.4638.
10
SOS induction by thermosensitive replication mutants of miniF plasmid.微小F质粒的热敏复制突变体诱导SOS反应。
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引用本文的文献

1
Isolation and characterization of lambda phages carrying the basic replicon of the resistance plasmid R1.携带耐药质粒R1基本复制子的λ噬菌体的分离与鉴定
Mol Gen Genet. 1981;182(1):148-53. doi: 10.1007/BF00422782.
2
DNA fusion product of phage P2 with plasmid pBR322: a new phasmid.噬菌体P2与质粒pBR322的DNA融合产物：一种新的噬菌粒。
Mol Gen Genet. 1983;189(2):343-7. doi: 10.1007/BF00337829.
3
Mini-F encoded proteins: identification of a new 10.5 kilodalton species.微小F编码蛋白：一种新的10.5千道尔顿蛋白的鉴定

本文引用的文献

1
Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination.大肠杆菌K-12中DNA修复和基因重组存在缺陷的突变体。
Genetics. 1966 Jun;53(6):1137-50. doi: 10.1093/genetics/53.6.1137.
2
Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.在与一类大肠杆菌K12 Rec-受体菌株交配时形成部分二倍体。
Proc Natl Acad Sci U S A. 1968 May;60(1):160-7. doi: 10.1073/pnas.60.1.160.
3
Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.
EMBO J. 1983;2(11):1853-61. doi: 10.1002/j.1460-2075.1983.tb01671.x.
4
Ham22, a mini-F mutation which is lethal to host cell and promotes recA-dependent induction of lambdoid prophage.Ham22，一种微小F突变体，对宿主细胞具有致死性，并促进λ样原噬菌体的recA依赖性诱导。
EMBO J. 1983;2(11):1863-8. doi: 10.1002/j.1460-2075.1983.tb01672.x.
5
Cleavage of lambda repressor and synthesis of RecA protein induced by transferred UV-damaged F sex factor.转移的紫外线损伤F性因子诱导λ阻遏物的裂解和RecA蛋白的合成。
Mol Gen Genet. 1982;186(2):170-9. doi: 10.1007/BF00331847.
6
Mini-F plasmid-induced SOS signal in Escherichia coli is RecBC dependent.大肠杆菌中微小F质粒诱导的SOS信号依赖于RecBC。
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5973-7. doi: 10.1073/pnas.82.17.5973.
7
The 41 carboxy-terminal residues of the miniF plasmid CcdA protein are sufficient to antagonize the killer activity of the CcdB protein.微小F质粒CcdA蛋白的41个羧基末端残基足以拮抗CcdB蛋白的杀伤活性。
Mol Gen Genet. 1991 Apr;226(1-2):297-304. doi: 10.1007/BF00273616.
质粒不相容性与复制控制：大肠杆菌K-12中R因子R1的复制突变体
J Bacteriol. 1975 Nov;124(2):641-9. doi: 10.1128/jb.124.2.641-649.1975.
4
Cloning, isolation, and characterization of replication regions of complex plasmid genomes.复杂质粒基因组复制区域的克隆、分离及特性分析
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242-6. doi: 10.1073/pnas.72.6.2242.
5
Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.分离细菌复制子复制区域的方法：微型F'kn质粒的构建
J Bacteriol. 1976 Aug;127(2):982-7. doi: 10.1128/jb.127.2.982-987.1976.
6
F plasmid incompatibility and copy number genes: their map locations and interactions.
Plasmid. 1978 Sep;1(4):492-507. doi: 10.1016/0147-619x(78)90007-0.
7
Physical mapping of a DNA sequence common to plasmids of incompatibility group F I.不相容群F I质粒共有的一段DNA序列的物理图谱
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1190-4. doi: 10.1073/pnas.74.3.1190.
8
Lambdoid phages that simplify the recovery of in vitro recombinants.简化体外重组体回收的λ噬菌体。
Mol Gen Genet. 1977 Jan 7;150(1):53-61. doi: 10.1007/BF02425325.