Murray N E, Brammar W J, Murray K
Mol Gen Genet. 1977 Jan 7;150(1):53-61. doi: 10.1007/BF02425325.
Derivatives of phage lambda are described for use as vectors for fragments of DNA generated with the HindIII and EcoRI restriction enzymes. With some vectors, hybrid molecules are recognised by a change from a turbid to a clear plaque morphology resulting from the insertion of a fragment of DNA into the lambda gene coding for the phage regressor. Other vectors contain a central, replaceable fragment of DNA which imparts a readily recognisable phenotype. This central fragment may include either a gene for a mutant transfer RNA (suppressor) or a part of the lacZ gene of E. coli able to complement a lacZ host. The appropriate lacZ host and indicator plates permit the ready distinction between recombinant and vector phages by the colour of the plaques.
描述了噬菌体λ的衍生物用作由HindIII和EcoRI限制酶产生的DNA片段的载体。对于一些载体，由于将DNA片段插入到编码噬菌体回归蛋白的λ基因中，杂交分子会通过从浑浊噬菌斑形态变为清晰噬菌斑形态而被识别。其他载体含有一个中央可替换的DNA片段，该片段赋予一种易于识别的表型。这个中央片段可以包括一个突变转移RNA（抑制子）基因或大肠杆菌lacZ基因的一部分，能够互补lacZ宿主。合适的lacZ宿主和指示平板允许通过噬菌斑的颜色轻松区分重组噬菌体和载体噬菌体。
