Instituto de Investigación Sanitaria Princesa, Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Madrid, Spain.
Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
Sci Rep. 2017 Jun 14;7(1):3508. doi: 10.1038/s41598-017-03689-7.
microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.
microRNAs (miRNAs) 在 T 淋巴细胞激活过程中受到严格调控，以实现精确的免疫反应。在这里，我们分析了 T 细胞在与树突状细胞的同源相互作用下被激活时，miRNA 谱的变化。我们还研究了在 T 细胞激活中受调控的 miRNA 所共有的 mRNA 靶标。pik3r1 基因，编码 PI3K p50、p55 和 p85 的调节亚基，被鉴定为 T 细胞激活后上调的 miRNA 的靶标。我们使用 3'UTR 荧光素酶报告基因和生化测定，显示了 miR-132-3p 上调和 pik3r1 基因表达之间的抑制关系。我们的结果表明，在 T 细胞激活过程中表达受到调控的特定 miRNA 可能调节 T 细胞中的 PI3K 信号。
