Singh Navdeep, Bansal Yashika, Bhandari Ranjana, Marwaha Lovish, Singh Raghunath, Chopra Kanwaljit, Kuhad Anurag
Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences, UGC-Centre of Advanced Study, Panjab University, Chandigarh, 160 014 India.
J Inflamm (Lond). 2017 Jun 9;14:14. doi: 10.1186/s12950-017-0158-3. eCollection 2017.
Indeed, intracerebral hemorrhage (ICH) account for only 15% of all strokes but it is one of the most devastating subtype of stroke associated with behavioral, cognitive and neurological deficits. The primary cause of neurological deficits in ICH is the hematoma growth, generation of free radicals, inflammatory cytokines and exhausting endogenous anti-oxidant machinery. It has been found that neuroinflammation following ICH leads to exaggeration of hallmarks of ICH. With this background, the study was aimed to evaluate the protective effect of resveratrol (RSV) in intracerebroventricular (ICV) collagenase (COL) induced neurological deficits in rats.
The present study was designed to explore the protective effects of resveratrol (5, 10, 20 mg/kg) against ICV-COL induced ICH. Animals were subjected to a battery of behavioral tests to access behavioral changes, including neurological scoring tests (cylinder test, spontaneous motility, righting reflex, horizontal bar test, forelimb flexion), actophotometer, rotarod, Randall Sellito and von Frey. Post stroke depression was estimated using forced swim test (FST). Memory deficit was monitored using Morris water maze (MWM).
Chronic treatment with RSV (20 mg/kg) for 21 days restored various behavioral changes, including neurological scoring tests (cylinder test, spontaneous motility, righting reflex, horizontal bar test, forelimb flexion), actophotometer, rotarod, Randall Sellito and Von Frey. RSV also restores increase in immobility time forced swim test used to evaluate post stroke depression and impaired memory deficit in Morris water maze. RSV administration also attenuated increased nitro-oxidative stress and TNF-α level. RSV being a potent antioxidant also restores changes in endogenous anti-oxidant levels.
In conclusion, our research demonstrates that RSV has a protective effect against ICH by virtue of its anti-inflammatory property and antioxidant and nitrosative stress restoring property.
事实上，脑出血（ICH）仅占所有中风的15%，但它是与行为、认知和神经功能缺损相关的最具破坏性的中风亚型之一。脑出血导致神经功能缺损的主要原因是血肿扩大、自由基生成、炎性细胞因子以及内源性抗氧化机制耗竭。已发现脑出血后的神经炎症会加剧脑出血的特征。在此背景下，本研究旨在评估白藜芦醇（RSV）对大鼠脑室内（ICV）注射胶原酶（COL）诱导的神经功能缺损的保护作用。
本研究旨在探讨白藜芦醇（5、10、20mg/kg）对ICV-COL诱导的脑出血的保护作用。对动物进行一系列行为测试以评估行为变化，包括神经评分测试（圆筒试验、自发运动、翻正反射、单杠试验、前肢屈曲）、光电计、转棒试验、 Randall Sellito试验和von Frey试验。使用强迫游泳试验（FST）评估中风后抑郁。使用莫里斯水迷宫（MWM）监测记忆缺陷。
RSV（20mg/kg）连续21天的慢性治疗恢复了各种行为变化，包括神经评分测试（圆筒试验、自发运动、翻正反射、单杠试验、前肢屈曲）、光电计、转棒试验、 Randall Sellito试验和Von Frey试验。RSV还恢复了用于评估中风后抑郁的强迫游泳试验中不动时间的增加以及莫里斯水迷宫中受损的记忆缺陷。RSV给药还减轻了硝基氧化应激和TNF-α水平的升高。RSV作为一种有效的抗氧化剂，还恢复了内源性抗氧化剂水平的变化。
总之，我们的研究表明，RSV凭借其抗炎特性以及抗氧化和亚硝化应激恢复特性，对脑出血具有保护作用。
