Talwar Brendan, Bouyoucos Ian A, Shipley Oliver, Rummer Jodie L, Mandelman John W, Brooks Edward J, Grubbs R Dean
Coastal and Marine Laboratory, Florida State University, St. Teresa, FL 32358, USA.
Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Conserv Physiol. 2017 Feb 27;5(1):cox012. doi: 10.1093/conphys/cox012. eCollection 2017.
Quantifying changes in blood chemistry in elasmobranchs can provide insights into the physiological insults caused by anthropogenic stress, and can ultimately inform conservation and management strategies. Current methods for analysing elasmobranch blood chemistry in the field are often costly and logistically challenging. We compared blood pH values measured using a portable, waterproof pH meter (Hanna Instruments HI 99161) with blood pH values measured by an i-STAT system (CG4+ cartridges), which was previously validated for teleost and elasmobranch fishes, to gauge the accuracy of the pH meter in determining whole blood pH for the Cuban dogfish () and lemon shark (). There was a significant linear relationship between values derived the pH meter and the i-STAT for both species across a wide range of pH values and temperatures (Cuban dogfish: 6.8-7.1 pH 24-30°C; lemon sharks: 7.0-7.45 pH 25-31°C). The relative error in the pH meter's measurements was ~±2.7%. Using this device with appropriate correction factors and consideration of calibration temperatures can result in both a rapid and accurate assessment of whole blood pH, at least for the two elasmobranch species examined here. Additional species should be examined in the future across a wide range of temperatures to determine whether correction factors are universal.
量化板鳃亚类动物血液化学成分的变化可以深入了解人为压力所造成的生理损伤，并最终为保护和管理策略提供依据。目前在野外分析板鳃亚类动物血液化学成分的方法往往成本高昂且在后勤方面具有挑战性。我们将使用便携式防水pH计（Hanna Instruments HI 99161）测得的血液pH值与通过i-STAT系统（CG4 +试剂盒）测得的血液pH值进行了比较，该系统先前已在硬骨鱼和板鳃亚类鱼类中得到验证，以评估pH计在测定古巴角鲨（）和柠檬鲨（）全血pH值时的准确性。在广泛的pH值和温度范围内（古巴角鲨：pH值6.8 - 7.1，温度24 - 30°C；柠檬鲨：pH值7.0 - 7.45，温度25 - 31°C），两种鲨鱼通过pH计和i-STAT测得的值之间均存在显著的线性关系。pH计测量的相对误差约为±2.7%。使用该设备并结合适当的校正因子并考虑校准温度，至少对于此处研究的两种板鳃亚类动物而言，能够快速且准确地评估全血pH值。未来应在更广泛的温度范围内对更多物种进行研究，以确定校正因子是否具有通用性。
