• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一项用于估计蚊虫吸食血餐后进食时间的人类基因分型试验。

A human genotyping trial to estimate the post-feeding time from mosquito blood meals.

作者信息

Hiroshige Yuuji, Hara Masaaki, Nagai Atsushi, Hikitsuchi Tomoyuki, Umeda Mitsuo, Kawajiri Yumi, Nakayama Koji, Suzuki Koichi, Takada Aya, Ishii Akira, Yamamoto Toshimichi

机构信息

Department of Legal Medicine and Bioethics, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan.

Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, Japan.

出版信息

PLoS One. 2017 Jun 15;12(6):e0179319. doi: 10.1371/journal.pone.0179319. eCollection 2017.

DOI:10.1371/journal.pone.0179319
PMID:28617865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5472291/
Abstract

Mosquitoes occur almost worldwide, and females of some species feed on blood from humans and other animals to support ovum maturation. In warm and hot seasons, such as the summer in Japan, fed mosquitoes are often observed at crime scenes. The current study attempted to estimate the time that elapsed since feeding from the degree of human DNA digestion in mosquito blood meals and also to identify the individual human sources of the DNA using genotyping in two species of mosquito: Culex pipiens pallens and Aedes albopictus. After stereomicroscopic observation, the extracted DNA samples were quantified using a human DNA quantification and quality control kit and were genotyped for 15 short tandem repeats using a commercial multiplexing kit. It took about 3 days for the complete digestion of a blood meal, and genotyping was possible until 2 days post-feeding. The relative peak heights of the 15 STRs and DNA concentrations were useful for estimating the post-feeding time to approximately half a day between 0 and 2 days. Furthermore, the quantitative ratios derived from STR peak heights and the quality control kit (Q129/Q41, Q305/Q41, and Q305/Q129) were reasonably effective for estimating the approximate post-feeding time after 2-3 days. We suggest that this study may be very useful for estimating the time since a mosquito fed from blood meal DNA, although further refinements are necessary to estimate the times more accurately.

摘要

蚊子几乎遍布全球，一些种类的雌蚊以人类和其他动物的血液为食以促进卵子成熟。在温暖和炎热的季节，比如日本的夏季，经常会在犯罪现场发现吸食过血液的蚊子。本研究试图根据蚊子血餐中人类DNA的消化程度来估算进食后经过的时间，并通过基因分型来确定两种蚊子（淡色库蚊和白纹伊蚊）血餐中人类DNA的个体来源。经过体视显微镜观察后，使用人类DNA定量和质量控制试剂盒对提取的DNA样本进行定量，并使用商业多重试剂盒对15个短串联重复序列进行基因分型。一顿血餐完全消化大约需要3天时间，进食后2天内都可以进行基因分型。15个短串联重复序列的相对峰高和DNA浓度有助于在0至2天内将进食后时间估算到大约半天。此外，从短串联重复序列峰高和质量控制试剂盒得出的定量比率（Q129/Q4l、Q305/Q4l和Q305/Q129）对于估算进食后2至3天的大致时间相当有效。我们认为，尽管要更准确地估算时间还需要进一步完善，但本研究对于根据蚊子血餐DNA估算进食时间可能非常有用

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/30ef066aebff/pone.0179319.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/5e73ec6eaf13/pone.0179319.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/fb1d506f115f/pone.0179319.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/214cdfc2a1d5/pone.0179319.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/dc74082b3633/pone.0179319.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/af43cb74c77e/pone.0179319.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/a0e492c5405e/pone.0179319.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/02cd7df55859/pone.0179319.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/b877154dc80a/pone.0179319.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/6d6b5b97ed2b/pone.0179319.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/48889b57dc1c/pone.0179319.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/30ef066aebff/pone.0179319.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/5e73ec6eaf13/pone.0179319.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/fb1d506f115f/pone.0179319.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/214cdfc2a1d5/pone.0179319.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/dc74082b3633/pone.0179319.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/af43cb74c77e/pone.0179319.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/a0e492c5405e/pone.0179319.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/02cd7df55859/pone.0179319.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/b877154dc80a/pone.0179319.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/6d6b5b97ed2b/pone.0179319.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/48889b57dc1c/pone.0179319.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/163a/5472291/30ef066aebff/pone.0179319.g011.jpg

相似文献

1
A human genotyping trial to estimate the post-feeding time from mosquito blood meals.一项用于估计蚊虫吸食血餐后进食时间的人类基因分型试验。
PLoS One. 2017 Jun 15;12(6):e0179319. doi: 10.1371/journal.pone.0179319. eCollection 2017.
2
A fast and inexpensive genotyping system for the simultaneous analysis of human and Aedes albopictus short tandem repeats.一种快速且廉价的基因分型系统，用于同时分析人类和白纹伊蚊短串联重复序列。
Parasit Vectors. 2023 Oct 5;16(1):347. doi: 10.1186/s13071-023-05977-w.
3
Forensic DNA Analysis of Mixed Mosquito Blood Meals: STR Profiling for Human Identification.混合蚊虫血餐的法医DNA分析：用于人类身份识别的STR分型
Insects. 2023 May 16;14(5):467. doi: 10.3390/insects14050467.
4
Forensic analysis of mosquito blood meal digestion process and the impact of heroin opiate: determination of the post-feeding interval as a PMI estimation.蚊虫血食消化过程及海洛因鸦片影响的法医分析：作为 PMI 估计的饲后时间的确定。
J Med Entomol. 2024 Jan 12;61(1):74-86. doi: 10.1093/jme/tjad153.
5
Repellent and insecticidal efficacy of a new combination of fipronil and permethrin against three mosquito species (Aedes albopictus, Aedes aegypti and Culex pipiens) on dogs.氟虫腈与氯菊酯新组合对犬只身上三种蚊虫（白纹伊蚊、埃及伊蚊和致倦库蚊）的驱避及杀虫效果
Parasit Vectors. 2015 Jan 30;8:64. doi: 10.1186/s13071-015-0691-y.
6
Host-feeding habits of Culex pipiens and Aedes albopictus (Diptera: Culicidae) collected at the urban and suburban residential areas of Japan.在日本城市和郊区居民区采集的库蚊和白纹伊蚊（双翅目：蚊科）的宿主取食习性。
J Med Entomol. 2010 May;47(3):442-50. doi: 10.1603/ME09256.
7
Temporal Gene Expression Profiles of Pre Blood-Fed Adult Females Immediately Following Eclosion in the Southern House Mosquito Culex Quinquefasciatus.刚羽化后未吸血成年雌性南方家蚊（致倦库蚊）的时间基因表达谱
Int J Biol Sci. 2015 Sep 20;11(11):1306-13. doi: 10.7150/ijbs.12829. eCollection 2015.
8
A comparision of West Nile Virus transmission by Ochlerotatus trivittatus (COQ.), Culex pipiens (L.), and Aedes albopictus (Skuse).三带喙库蚊（COQ.）、致倦库蚊（L.）和白纹伊蚊（Skuse）传播西尼罗河病毒的比较
Vector Borne Zoonotic Dis. 2005 Spring;5(1):40-7. doi: 10.1089/vbz.2005.5.40.
9
Susceptibility of Ochlerotatus trivittatus (Coq.), Aedes albopictus (Skuse), and Culex pipiens (L.) to West Nile virus infection.三带喙库蚊（Coq.）、白纹伊蚊（Skuse）和致倦库蚊（L.）对西尼罗河病毒感染的易感性。
Vector Borne Zoonotic Dis. 2004 Fall;4(3):190-7. doi: 10.1089/vbz.2004.4.190.
10
Detecting multiple DNA human profile from a mosquito blood meal.从蚊子吸食的血液中检测多种人类DNA图谱。
Genet Mol Res. 2016 Aug 26;15(3):gmr7547. doi: 10.4238/gmr.15037547.

引用本文的文献

1
A fast and inexpensive genotyping system for the simultaneous analysis of human and Aedes albopictus short tandem repeats.一种快速且廉价的基因分型系统，用于同时分析人类和白纹伊蚊短串联重复序列。
Parasit Vectors. 2023 Oct 5;16(1):347. doi: 10.1186/s13071-023-05977-w.
2
Forensic DNA Analysis of Mixed Mosquito Blood Meals: STR Profiling for Human Identification.混合蚊虫血餐的法医DNA分析：用于人类身份识别的STR分型
Insects. 2023 May 16;14(5):467. doi: 10.3390/insects14050467.
3
Identity of the numerous bloodstains at the murder scene: molecular identification of fly artifacts and fly species by CO1 analysis.

本文引用的文献

1
Avian malaria parasites in the last supper: identifying encounters between parasites and the invasive Asian mosquito tiger and native mosquito species in Italy.“最后的晚餐”中的鸟类疟原虫：确定意大利境内寄生虫与入侵的亚洲虎蚊及本地蚊种之间的接触情况。
Malar J. 2015 Jan 28;14:32. doi: 10.1186/s12936-015-0571-0.
2
Identification of person and quantification of human DNA recovered from mosquitoes (Culicidae).从蚊子（蚊科）中鉴定人和定量人 DNA。
Forensic Sci Int Genet. 2014 Jan;8(1):109-12. doi: 10.1016/j.fsigen.2013.07.011. Epub 2013 Sep 7.
3
Effect of blood meal digestion and DNA extraction protocol on the success of blood meal source determination in the malaria vector Anopheles atroparvus.
谋杀现场众多血迹的鉴定：通过细胞色素氧化酶亚基I（CO1）分析对苍蝇痕迹和苍蝇种类进行分子鉴定。
Int J Legal Med. 2023 May;137(3):835-842. doi: 10.1007/s00414-022-02939-2. Epub 2022 Dec 21.
4
Determination of human identity from Anopheles stephensi mosquito blood meals using direct amplification and massively parallel sequencing.利用直接扩增和大规模并行测序技术从致倦库蚊血餐中鉴定人类身份。
Forensic Sci Int Genet. 2020 Sep;48:102347. doi: 10.1016/j.fsigen.2020.102347. Epub 2020 Jul 11.
5
Louse flies of Eleonora's falcons that also feed on their prey are evolutionary dead-end hosts for blood parasites.以埃利奥诺拉的隼类的虱蝇为食的血寄生虫，在进化上是它们的死胡同宿主。
Mol Ecol. 2019 Apr;28(7):1812-1825. doi: 10.1111/mec.15020. Epub 2019 Apr 4.
血液消化和 DNA 提取方法对疟蚊 Anopheles atroparvus 血食源分析的影响。
Malar J. 2013 Mar 21;12:109. doi: 10.1186/1475-2875-12-109.
4
Analysis of post-blood meal flight distances in mosquitoes utilizing zoo animal blood meals.利用动物园动物血餐对蚊子血餐后飞行距离的分析。
J Vector Ecol. 2012 Jun;37(1):83-9. doi: 10.1111/j.1948-7134.2012.00203.x.
5
Blood-feeding ecology of mosquitoes in zoos.动物园中蚊子的吸血生态学
Med Vet Entomol. 2012 Dec;26(4):407-16. doi: 10.1111/j.1365-2915.2012.01012.x. Epub 2012 Mar 6.
6
Analysis of mosquito bloodmeals using RFLP markers.使用限制性片段长度多态性（RFLP）标记分析蚊子血餐。
Exp Parasitol. 2006 Dec;114(4):259-64. doi: 10.1016/j.exppara.2006.04.001. Epub 2006 May 22.
7
Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction.利用二阶导数计算和双重校正的改进型实时逆转录聚合酶链反应高通量测量方法。
Biotechniques. 2005 Feb;38(2):287-93. doi: 10.2144/05382RR05.
8
Age-grouping methods in Diptera of medical importance with special reference to some vectors of malaria.具有医学重要性的双翅目昆虫的年龄分组方法，特别提及一些疟疾传播媒介。
Monogr Ser World Health Organ. 1962;47:13-191.
9
Identification of mosquito bloodmeals using polymerase chain reaction (PCR) with order-specific primers.使用针对特定目的的引物通过聚合酶链反应（PCR）鉴定蚊虫血餐。
J Med Entomol. 2003 Mar;40(2):215-22. doi: 10.1603/0022-2585-40.2.215.
10
Extent of digestion affects the success of amplifying human DNA from blood meals of Anopheles gambiae (Diptera: Culicidae).消化程度会影响从冈比亚按蚊（双翅目：蚊科）血餐中扩增人类DNA的成功率。
Bull Entomol Res. 2002 Jun;92(3):233-9. doi: 10.1079/BER2002164.