He Xia, Ji Jun, Wang Tao, Wang Ming-Bang, Chen Xiao-Ling
Department of Pathology, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen, China.
Cardiology. 2017;138(2):107-114. doi: 10.1159/000476029. Epub 2017 Jun 16.
Many circulating microRNAs (miRs) have been shown to have potential biomarker effects in cardiovascular disease. We studied the dysregulation of circulating miR-195-3p in patients with heart failure (HF) to elucidate its value as a potential biomarker for HF.
Eight ischemic HF (IHF) patients, 8 nonischemic HF patients (NIHF), and 8 healthy volunteers (matched by age and sex - normal controls [NCs]) were chosen for miR sequencing. The plasma RNA was extracted, and a small RNA library of HF was established and then sequenced using next-generation sequencing (NGS) technology. The miR-195-3p was selected for a second clinical study in 60 IHF, 48 NIHF patients, and 35 NCs for qRT-PCR validation.
The expression of circulating miR-195-3p in the IHF group was increased 69.5-fold compared with the NC group using NGS technique, and it was the most elevated in all upregulated miRs. MiR-195-3p was ranked in the top 1 of all upregulated miRs in contribution to HF based on a random forest model analysis. The upregulation of circulating miR-195-3p was also validated with the qRT-PCR method, and receiver operating characteristic curve analysis showed that the area under the curve (AUC) was 0.831.
The circulating miR-195-3p was upregulated in IHF and NIHF patients and could be a potential biomarker for HF.
许多循环微RNA（miR）已被证明在心血管疾病中具有潜在的生物标志物作用。我们研究了心力衰竭（HF）患者循环miR-195-3p的失调情况，以阐明其作为HF潜在生物标志物的价值。
选取8例缺血性HF（IHF）患者、8例非缺血性HF患者（NIHF）和8名健康志愿者（按年龄和性别匹配 - 正常对照[NC]）进行miR测序。提取血浆RNA，建立HF的小RNA文库，然后使用下一代测序（NGS）技术进行测序。选择miR-195-3p在60例IHF、48例NIHF患者和35例NC中进行第二项临床研究，以进行qRT-PCR验证。
使用NGS技术，IHF组中循环miR-195-3p的表达与NC组相比增加了69.5倍，并且在所有上调的miR中它升高幅度最大。基于随机森林模型分析，miR-195-3p在所有上调的miR中对HF的贡献排名第一。循环miR-195-3p的上调也通过qRT-PCR方法得到验证，受试者工作特征曲线分析显示曲线下面积（AUC）为0.831。
循环miR-195-3p在IHF和NIHF患者中上调，可能是HF的潜在生物标志物。
