Borsanelli Ana C, Gaetti-Jardim Elerson, Schweitzer Christiane M, Viora Lorenzo, Busin Valentina, Riggio Marcello P, Dutra Iveraldo S
School of Veterinary Medicine, Universidade Estadual Paulista, Araçatuba, Brazil.
Dental School, Universidade Estadual Paulista, Araçatuba, Brazil.
Vet Microbiol. 2017 May;203:271-274. doi: 10.1016/j.vetmic.2017.03.032. Epub 2017 Mar 31.
Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melalinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures.
牙周炎是一种多微生物感染性疾病，可导致咬合改变、牙齿脱落、反刍困难以及动物过早淘汰。本研究旨在检测病变深度超过5毫米的绵羊（n = 14）牙周袋以及被认为牙周健康的动物（n = 20）牙龈沟中存在的卟啉单胞菌属和普氏菌属的菌种。使用针对不解糖卟啉单胞菌、牙髓卟啉单胞菌、牙龈卟啉单胞菌、龈沟卟啉单胞菌、颊普氏菌、中间普氏菌、洛氏普氏菌、产黑色素普氏菌、变黑普氏菌、口腔普氏菌和坦氏普氏菌的特异性引物，通过聚合酶链反应（PCR）评估微生物的存在。使用学生t检验和Spearman相关性进行患病率和风险分析。在绵羊牙周病变中检测到的普氏菌属和卟啉单胞菌属菌种中，产黑色素普氏菌（85.7%）、颊普氏菌（64.3%）、牙龈卟啉单胞菌（50%）和牙髓卟啉单胞菌（50%）最为常见。牙龈卟啉单胞菌（15%）和口腔普氏菌（10%）在牙龈沟中占主导。在所研究的34个样本中未检测到龈沟卟啉单胞菌和坦氏普氏菌。通过t检验进行的数据评估证实，不解糖卟啉单胞菌、牙髓卟啉单胞菌、牙龈卟啉单胞菌、颊普氏菌、中间普氏菌、产黑色素普氏菌和变黑普氏菌的存在与绵羊牙周炎相关。本研究结果将对绵羊牙周炎发病机制的研究及其控制措施的制定做出重要贡献。
