Department of Veterinary Medicine, School of Veterinary Medicine and Animal Science, Universidade Federal de Goiás (UFG), Goiania, Goias, Brazil.
Department of Production and Animal Health, School of Veterinary Medicine, São Paulo State University (Unesp), Araçatuba Campus, Sao Paulo, Brazil.
Microb Ecol. 2023 Jul;86(1):687-698. doi: 10.1007/s00248-022-02062-0. Epub 2022 Jul 2.
Periodontitis is a polymicrobial biofilm-induced inflammatory disease associated with a dysbiotic microbial community and severely affects the health and welfare of animals. However, little is known regarding the dental microbiota associated with this disease in goats. In this study, we used high-throughput sequencing, network analysis, and predicted functions to investigate the microbiota of clinically healthy goats and those with periodontitis and identify possible pathogens and proteins associated with the disease. Dental microbiomes of goats with periodontitis were richer, and network analyses showed that the number of negative interactions was higher in the networks of animals with periodontitis. Based on the interrelationships, Porphyromonas, Fusobacterium, and Prevotella were suggested to play an important role in the dental microbiota associated with goat periodontitis. Protein families linked to translation, cytoplasmatic translation, and rRNA processing were more abundant in the dental microbiota of goats with periodontitis. In conclusion, the dental biofilm microbiota associated with goat periodontitis seems to be dysbiotic and has significant antagonistic interactions, which discriminate healthy animals from diseased animals and highlight the importance of key bacteria. Thus, these novel findings contribute to the evolution of knowledge regarding the etiopathogenesis of goat periodontitis and possibly to the development of periodontitis control measures.
牙周炎是一种多微生物生物膜诱导的炎症性疾病，与微生物群落失调有关，严重影响动物的健康和福利。然而，关于山羊牙周炎相关的口腔微生物群，我们知之甚少。在这项研究中，我们使用高通量测序、网络分析和预测功能来研究临床健康山羊和牙周炎山羊的口腔微生物群，并确定与疾病相关的可能病原体和蛋白质。牙周炎山羊的口腔微生物群更丰富，网络分析表明，牙周炎动物网络中的负相互作用数量更高。基于相互关系，提示卟啉单胞菌、梭杆菌和普雷沃氏菌在与山羊牙周炎相关的口腔微生物群中起重要作用。与翻译、细胞质翻译和 rRNA 加工相关的蛋白质家族在牙周炎山羊的口腔微生物群中更为丰富。总之，与山羊牙周炎相关的口腔生物膜微生物群似乎失调，具有显著的拮抗相互作用，可将健康动物与患病动物区分开来，并强调关键细菌的重要性。因此，这些新发现有助于了解山羊牙周炎的病因发病机制的知识发展，并可能有助于牙周炎控制措施的发展。
