Dong Chuanfu, Wang Zhimei, Weng Shaoping, He Jianguo
State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, People's Republic of China; Bairong Aquatic Breeding Products Co., Ltd, Xiaan, Danzhao Town, Nanhai District, Foshan 528000, People's Republic of China.
State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Vet Microbiol. 2017 May;203:28-33. doi: 10.1016/j.vetmic.2017.02.006. Epub 2017 Feb 17.
A novel ranavirus with features similar to largemouth bass virus (LMBV) was isolated and then characterized from a natural mass mortality of adult hybrid mandarin (Siniperca scherzeri×Siniperca chuatsi). The isolated LMBV-like iridovirus was designated as mandarin ranavirus (MRV)-GD1301. The results of artificial infection showed that MRV-GD1301 was highly virulent to hybrid mandarin juveniles, and 100% mortality was observed within 5days after infection via intraperitoneal injection. Moribund fishes typically have abnormally swollen abdomens with extremely severe ascites and exhibit exophthalmia. The characteristic clinical signs have been rarely recorded in other LMBV-associated fish diseases and other viral diseases in mandarin aquaculture. In contrast to the high lethality in hybrid mandarin, MRV-GD1301 showed avirulence to koi Cyprinus carpio, a susceptible fish species to LMBV-like koi ranavirus (KIRV) found recently in India. Our findings suggest that MRV is an emerging causative agent of mass mortality in mandarin species.
从成年杂交鳜（斑鳜×翘嘴鳜）自然大规模死亡事件中分离出一种具有与大口黑鲈病毒（LMBV）相似特征的新型蛙病毒，随后对其进行了特征分析。分离出的LMBV样虹彩病毒被命名为鳜蛙病毒（MRV）-GD1301。人工感染结果表明，MRV-GD1301对杂交鳜幼鱼具有高致病性，通过腹腔注射感染后5天内观察到100%的死亡率。濒死鱼通常腹部异常肿胀，伴有极其严重的腹水，并出现眼球突出。在其他与LMBV相关的鱼类疾病和鳜养殖中的其他病毒性疾病中，很少记录到这些特征性临床症状。与在杂交鳜中的高致死率相反，MRV-GD1301对锦鲤（Cyprinus carpio）无毒力，锦鲤是最近在印度发现的对LMBV样锦鲤蛙病毒（KIRV）易感的鱼类品种。我们的研究结果表明，MRV是鳜类大规模死亡的一种新的致病因子。
