Kayansamruaj P, Rangsichol A, Dong H T, Rodkhum C, Maita M, Katagiri T, Pirarat N
Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand.
J Fish Dis. 2017 Oct;40(10):1341-1350. doi: 10.1111/jfd.12606. Epub 2017 Jan 23.
In 2013, an outbreak of ulcerative disease associated with ranavirus infection occurred in barcoo grunter, Scortum barcoo (McCulloch & Waite), farms in Thailand. Affected fish exhibited extensive haemorrhage and ulceration on skin and muscle. Microscopically, the widespread haemorrhagic ulceration and necrosis were noted in gill, spleen and kidney with the presence of intracytoplasmic eosinophilic inclusion bodies. When healthy barcoo grunter were experimentally challenged via intraperitoneal and oral modes with homogenized tissue of naturally infected fish, gross and microscopic lesions occurred with a cumulative mortality of 70-90%. Both naturally and experimentally infected fish yielded positive results to the ranavirus-specific PCR. The full-length nucleotide sequences of major capsid protein gene of ranaviral isolates were similar to largemouth bass virus (LMBV) and identical to largemouth bass ulcerative syndrome virus (LBUSV), previously reported in farmed largemouth bass (Micropterus salmoides L.), which also produced lethal ulcerative skin lesions. To the best of our knowledge, this is the first report of a LMBV-like infection associated with skin lesions in barcoo grunter, adding to the known examples of ranavirus infection associated with skin ulceration in fish.
2013年，泰国巴库鲈（Scortum barcoo，麦卡洛克和韦特）养殖场爆发了与蛙病毒感染相关的溃疡性疾病。患病鱼的皮肤和肌肉出现大面积出血和溃疡。显微镜下，鳃、脾脏和肾脏出现广泛的出血性溃疡和坏死，并伴有胞质内嗜酸性包涵体。当用自然感染鱼的匀浆组织通过腹腔内和口服方式对健康的巴库鲈进行实验性攻毒时，出现了肉眼可见和显微镜下的病变，累积死亡率为70%至90%。自然感染和实验感染的鱼对蛙病毒特异性PCR均呈阳性结果。蛙病毒分离株主要衣壳蛋白基因的全长核苷酸序列与大口黑鲈病毒（LMBV）相似，与之前在养殖大口黑鲈（Micropterus salmoides L.）中报道的大口黑鲈溃疡性综合征病毒（LBUSV）相同，后者也会导致致命的溃疡性皮肤病变。据我们所知，这是首次报道巴库鲈中与皮肤病变相关的类LMBV感染，为已知的鱼类中与皮肤溃疡相关的蛙病毒感染实例增添了内容。
