Física i Cristal•lografia de Materials i Nanomaterials and EmaS. Universitat Rovira i Virgili, Carrer Marcel•lí Domingo 1, 43007, Tarragona, Spain.
Departamento de Química Física e Inorgánica and EmaS, Universitat Rovira i Virgili, Carrer Marcel•lí Domingo 1, 43007, Tarragona, Spain.
Sci Rep. 2017 Jun 16;7(1):3677. doi: 10.1038/s41598-017-04033-9.
Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design and demonstrate a 3D-flow focusing microfluidic device, where all optics are integrated into the chip, for the fluorescence quantification of CTCs in real samples. To test the chip performance, two cell membrane targets, the epithelial cell adhesion molecule, EpCAM, and the receptor tyrosine-protein kinase, HER2, are selected. The efficiency of the platform is demonstrated on cell lines and in a variety of healthy donors and metastatic-breast cancer patients.
转移性癌症患者在连续的治疗周期中需要持续监测，以仔细评估其疾病的进展。重复进行活检非常具有侵入性，且并非总是可行。在此，我们设计并展示了一种 3D 流聚焦微流控装置，其中所有光学器件都集成在芯片上，用于对真实样本中的 CTC 进行荧光定量分析。为了测试芯片的性能，选择了两种细胞膜靶标，上皮细胞黏附分子 EpCAM 和受体酪氨酸蛋白激酶 HER2。该平台的效率在细胞系以及各种健康供体和转移性乳腺癌患者中得到了验证。
